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What is MMDF?
MMDF is an acronym for Multiple Mass Defect Filter. A new feature in the latest version of Thermo Scientific MetWorks™ Software, this post-acquisition data processing tool allows the user to combine the results from up to six different Mass Defect Filters (MDF).
In order to explain MMDF, one needs to first understand the terms mass defect and mass defect filter. The term “mass defect” originates from the fact that only the mono isotopic element 12 C, has an integer value for atomic weight, i.e. 12.000000.
Mass defect refers to the difference between the exact mass of an element (or a compound) and its closest integer value. It may be positive (larger than the nominal mass) or negative (smaller than the nominal mass).
Mass Defect Filter (MDF) refers to a post-acquisition data filtering technique based on the mass defect of the parent drug and its metabolites. In order to use MDF, it is critically important to use high mass accuracy data from high resolution analyses, i.e. LTQ Orbitrap™ hybrid series mass spectrometer.
Why is MMDF more useful than MDF?
The ability to apply MMDF allows users to capture metabolites from Phase I and Phase II specifically and concurrently. It also can, at the same time, capture metabolites from hydrolysis or N-dealkylation, even when the products from such processes have mass defects that are significantly different from the parent. These capabilities are not possible with the use of a single MDF. The results from MMDF are much more specific, and the analysis provides faster results.
Learn more about the use of MMDF in metabolite analyses:
> Download the MetWorks Brochure and the featured application: Using Multiple Mass Defect Filters and Higher Energy Collisional Dissociation on an LTQ Orbitrap XL for Fast, Sensitive and Accurate Metabolite ID
> See more metabolism solutions
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