Crosslinking Reagents

Cross-linking is the process of chemically joining two molecules by a covalent bond. Cross-linking reagents contain reactive ends to specific functional groups (primary amines, sulfhydryls, etc.) on proteins or other molecules. Several chemical groups that may be targets for reactions in proteins and peptides are readily available, allowing them to be easily conjugated and studied using cross-linking methods. Cross-linkers also are commonly used to modify nucleic acids, drugs, solid surfaces and other molecules.

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Bioconjugate Toolkit Reagents
The Bioconjugate Toolkit Reagents are a series of heterobifunctional crosslinkers that can be applied to almost any bioconjugation procedure. These crosslinkers contain an NHS ester and either a hydrazine/hydrazide or aldehyde group.

Deuterated Crosslinkers for Mass Spec Analysis
Chemical crosslinking, combined with high-resolution mass spectrometry, is a quick strategy to obtain three-dimensional structural data of proteins and protein complexes using small quantities of protein.

Heterobifunctional Crosslinkers
Heterobifunctional crosslinkers possess two or more different reactive groups that allow for sequential conjugations with specific functional groups of proteins, minimizing undesirable polymerization or self-conjugation.

Homobifunctional Crosslinkers
Homobifunctional crosslinkers have two identical reactive groups and are often used in a one-step chemical crosslinking procedure.

NHS and Sulfo-NHS
COOH-containing molecule will form an acyl amino ester with NHS or Sulfo-NHS using a carbodiimide; this molecule will react with primary or secondary amines, yielding a stable amide bond.