Reducing Agents

Solution and solid-phase reducing agents, such as TCEP, DTT and mercaptoethanol, for disulfide-containing peptides and proteins.

For more information or to purchase, please follow the links below.

2-Mercaptoethanol
2-Mercaptoethanol (b-mercaptoethanol) is a mild reducing agent that is ideal for cleaving disulfide bonds and generating thiols.

2-Mercaptoethylamine·HCl
Ideal reducing agent to dissociate divalent IgG to monovalent IgG by reducing disulfide bonds in the hinge region without separating heavy and light chains.

Bond-Breaker TCEP Solution, Neutral pH
Bond-Breaker TCEP is an odorless reducing agent in a stabilized solution for SDS-PAGE and other applications that typically use DTT or mercaptoethanol.

Cysteine•HCl
Useful as a sulfhydryl group standard or a mild reducing agent.

Dithiothreitol DTT
A pre-measured, dry, room temperature-stable aliquot of DTT sealed in a microtube. Make a 500 mM solution of DTT in seconds.

Ellman’s ReagentA simple, sensitive and reliable method for determining free sulfhydryl content in peptides and proteins.

Immobilized TCEP Disulfide Reducing Gel
An odorless, immobilized reducing agent that’s there when you want it and gone when you don’t! Reduce peptides or proteins without contaminating them with reducing agent.

N-Ethylmaleimide
Covalently blocks sulfhydryl groups at pH 6.5-7.5 and the reaction of the maleimide group with sulfhydryls can be monitored by measuring the decrease in absorbance at 300 nm.

Reduce-Imm Reducing Columns
Solid phase system for reducing peptides and proteins.

TCEP·HCl
TCEP is a potent, water-soluble, odorless reducing agent that is a popular alternative to DTT and mercaptoethanol.