Ask the Experts Answers

Q1: Can I use the Quantech Digital Filter Fluorometer for AOAC methods?

Thermo Fisher Scientific: Yes, several AOAC methods are designed for filter fluorometers, including the analysis of Histimine in Seafood. Some of the other methods that can be run include… See Complete Answer 

Q2: I have noticed some assays use chemiluminescence from compounds like firefly luciferase. Can I use a Quantech Digital Filter Fluorometer to perform chemiluminescence assays?

Thermo Fisher Scientific: Yes. The Quantech Digital Filter Fluorometer is ideal for chemiluminescence assays. The sensitive photomultiplier detector in the Quantech Digital Filter Fluorometer allows for superior sensitivity. Because chemiluminescence assays typically do not require a spectrum to provide accurate data, researchers can save thousands of dollars, compared to purchasing expensive, scanning instruments.

Q3: I need a fluorescence detector for my LC system; can I use a Quantech Digital Filter Fluorometer?

Thermo Fisher Scientific: Yes. The Quantech Digital Filter Fluorometer is available in a configuration specifically designed to function as a chromatography detector. This model has PEEK tubing inputs and an 8-microliter flow cell. Simply use the DC voltage output and an analog to digital converter to get the fluorescence data into your chromatography software.

Q4: How can I increase the sensitivity of my assay?

Thermo Fisher Scientific: Using a mirrored cuvette that has one side of the cuvette coated with a mirrored surface will increase sensitivity. By reflecting more fluorescence toward the detector, the mirrored surface allows light that normally scatters off in an opposite direction to be re-directed toward the detector. Collecting more light means more sensitivity for your assay.

Q5: Can I use the Quantech Digital Filter Fluorometer to do DNA, RNA, and protein concentration assays?

Thermo Fisher Scientific: Yes. The Quantech Digital Filter Fluorometer is a sensitive and quick way to measure DNA, RNA, and protein concentration using fluorescent dyes. These kits rely on standards and the calibration curve is built into the Quantech instrument. Quickly change from one assay to the next simply by changing filters.

Q6: Can I use the Quantech Digital Filter Fluorometer for intrinsic protein fluorescence measurements?

Thermo Fisher Scientific: Yes. The Quantech Digital Filter Fluorometer is available in a configuration specifically designed for measuring the fluorescence of tyrosine, tryptophan, and phenylalanine. A phosphorescent coated mercury lamp provides balanced excitation between 270 and 330 nm. The sensitive PMT detector allows for sensitive measurements.

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