Application Note - siRNA & QPCR

This application note shows an experimental workflow for gene silencing with Thermo Scientific Dharmacon Accell siRNA and mRNA detection using Thermo Scientific Verso QRT-PCR.

A variety of alternative vehicles or delivery modes have been developed specifically for difficult-to-transfect cell types. The Dharmacon Accell siRNA technology represents one such innovation that successfully combines bioinformatics and chemical modification strategies to provide functional and specific siRNAs that are enhanced for delivery in the absence of lipid.

Quantitative RT-PCR (QRT-PCR) represents the most commonly recommended method for mRNA detection. However, QRT-PCR methods often suffer from variability and poor reproducibility. This application note also describes how the Verso™ QRTPCR SYBR® Green kits can be used to eliminate variability in detection - giving reliable and consistent mRNA level assessments.