Common Fluorescent Dyes
|
| Fluorphore/Dye |
Excitation Wavelength, nm |
Emission Wavelength, nm |
Excitation Filter |
Emission Filter |
| GENERAL USE |
| 2-amino purine |
308 |
367 |
LE1095X4 (308-318nm) |
LE1095x30 (340-380nm) |
| AlexaFluor® 488 |
495 |
519 |
LE1095X13 (485-495nm) |
LE1095X23 (≥515nm) |
| AlexaFluor® 532 |
532 |
554 |
LE1095x14 (515x525nm) |
LE1095X25 (≥550nm) |
| AlexaFluor® 546 |
556 |
573 |
LE1095x32 (563x573nm) |
LE1095X26 (≥585nm) |
| AlexaFluor® 610 |
612 |
628 |
LE1095x16 (585x595nm) |
LE1095X27 (≥605nm) |
| Cy™ 2 |
489 |
506 |
LE1095X13 (485-495nm) |
LE1095X22 (≥500nm) |
| Cy™ 3 |
550 |
570 |
LE1095x15 (535-545nm) |
LE1095X26 (≥585nm) |
| Cy™ 3B |
558 |
572 |
LE1095x32 (563-573nm) |
LE1095X26 (≥585nm) |
| Cy™ 3.5 |
581 |
596 |
LE1095X35 (572-582nm) |
LE1095X26 (≥585nm) |
| Fluorescein |
494 |
518 |
LE1095X13 (485-495nm) |
LE1095X23 (≥515nm) |
| Lanthanides (Europium) |
365 |
620 |
LE1095X6 (360-370nm) |
LE1095X27 (≥605nm) |
| Tetramethylrhodamine (TMR) |
555 |
580 |
LE1095X15 (535-545nm) |
LE1095X25 (≥550nm) |
| Texas Red® |
595 |
615 |
LE1095X16 (585-595nm) |
LE1095X27 (≥605nm) |
| CELL VIABILITY |
| alamarBlue™ (Resazurin) |
560 |
590 |
LE1095X32 (563-573nm) |
LE1095X26 (≥585nm) |
| propidium iodide |
530 |
615 |
LE1095X14 (515-525nm) |
LE1095X27 (≥605nm) |
| STYO® 9 |
483 |
503 |
LE1095X13 (485-495nm) |
LE1095X22 (≥500nm) |
| STYOX® Green |
504 |
523 |
LE1095x13 (485-495nm) |
LE1095X23 (≥515nm) |
| CYCLE 3 GFP QUANTITATION |
| Cycle 3 GFP |
395 |
507 |
LE1095X7 (385-395nm) |
LE1095X22 (≥500nm) |
| ENZYME QUANTITATION |
| 6,8-difluoro-4-methylumbelliferyl phosphate (DiFMUP) |
358 |
455 |
LE1095X30 (340-380nm) |
LE1095X20 (≥450nm) |
| 6,8-difluoro-7-hydroxy-4-methylcoumarin |
358 |
450 |
LE1095X30 (340-380nm) |
LE1095X20 (≥450nm) |
| 7-amino-4-methylcoumarin (AMC) |
345 |
445 |
LE1095X33 (335-345nm) |
LE1095X19 (≥430nm) |
| 7-hydroxy-4-methylcoumarin (ß-methylumbelliferone) |
360 |
455 |
LE1095X6 (360-370nm) |
LE1095X20 (≥450nm) |
| 7-hydroxycoumarin (umbelliferone) |
330 |
440 |
LE1095X33 (335-345nm) |
LE1095X19 (≥430nm) |
| Amplex® UltraRed |
530 |
580 |
LE1095X15 (535-545nm) |
LE1095X25 (≥550nm) |
| NAD/NADH |
340 |
460 |
LE1095X33 (335-345nm) |
LE1095X20 (≥450nm) |
| Resorufin |
570 |
585 |
LE1095X32 (563-573nm) |
LE1095X26 (≥585nm) |
| Rhodamine 110 |
496 |
520 |
LE1095X13 (485-495nm) |
LE1095X23 (≥515nm) |
| LIPIDS & LIPID METABOLITES |
| Amplex® Red |
530-560 |
590 |
LE1095X15 (535-545nm) |
LE1095X26 (≥585nm) |
| NUCLEIC ACIDS (DNA & RNA) QUANTITATION |
| 4'-6-diamidino-2-phenylindole (DAPI) |
360 |
453 |
LE1095X6 (360-370nm) |
LE1095X20 (≥450nm) |
| Ethidium Bromide |
302 or 546 |
590 |
LE1095X3 (292-302nm) or LE1095X15 (535-545nm) |
LE1095X26 (≥585nm) |
| Hoechst® 33258 |
365 |
460 |
LE1095X6 (360-370nm) |
LE1095X20 (≥450nm) |
| OliGreen® |
480 |
520 |
LE1095X13 (485-495nm) |
LE1095X23 (≥515nm) |
| PicoGreen® |
480 |
520 |
LE1095X13 (485-495nm) |
LE1095X23 (≥515nm) |
| RiboGreen® |
500 |
525 |
LE1095X13 (485-495nm) |
LE1095X23 (≥515nm) |
| SYBR® Gold |
495 |
537 |
LE1095X13 (485-495nm) |
LE1095X24 (≥535nm) |
| SYBR® Green I |
479 |
520 |
LE1095X31 (385-475nm) |
LE1095X23 (≥515nm) |
| TOTO® -1 |
514 |
533 |
LE1095X14 (515-525nm) |
LE1095X24 (≥535nm) |
| YO-PRO® -1 |
491 |
509 |
LE1095X13 (485-495nm) |
LE1095X22 (≥500nm) |
| YOYO® -1 |
491 |
509 |
LE1095X13 (485-495nm) |
LE1095X22 (≥500nm) |
| OXIDASES |
| Amplex® Red |
530-560 |
590 |
LE1095X15 (535-545nm) |
LE1095X26 (≥585nm) |
| PROTEIN QUANTITATION |
| Albumin Blue |
560 |
620 |
LE1095X32 (563-573nm) |
LE1095X27 (≥605nm) |
| ATTO-TAG™ |
485 |
530 |
LE1095X13 (485-495nm) |
LE1095X23 (≥515nm) |
| Epicocconone |
390 |
620 |
LE1095X7 (385-395nm) |
LE1095X27 (≥605nm) |
| Fluorescamine |
380 |
464 |
LE1095X7 (385-395nm) |
LE1095X20 (≥450nm) |
| NanoOrange® |
485 |
590 |
LE1095X13 (485-495nm) |
LE1095X26 (≥585nm) |
| o-phthaldialdehyde; o-phthalaldehyde (OPA) |
334 |
455 |
LE1095X33 (335-345nm) |
LE1095X20 (≥450nm) |
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