Fractionate subcellular proteins for localization studies Segregate and enrich proteins from five cellular compartments
The Thermo Scientific Subcellular Protein Fractionation Kit enables the stepwise separation and extraction of cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal proteins from mammalian cultured cells or tissue. Extracts obtained with the Subcellular Protein Fractionation Kit are compatible with a variety of downstream applications including Western blotting, protein assays, electrophoretic mobility shift assays and reporter-gene and enzyme-activity assays.
Fractionation of subcellular proteins enables protein localization assessment and protein enrichment from specific cellular compartments. The Subcellular Protein Fractionation Kit includes a combination of reagents for stepwise lysis of cells into functional cytoplasmic, membrane, nuclear soluble, chromatin-bound and cytoskeletal protein fractions in less than 3 hours. Extracts from each subcellular compartment generally have less than 15% contamination between fractions, which is sufficient purity for most experiments studying protein localization and redistribution.
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Fractionate subcellular proteins for localization studies