Capture, purify and identify specific tyrosine phorphorylated proteins using your choice of specific Src Homology 2 domains (SH2 domains)
Thermo Scientific SH2 Domain Phosphotyrosine Capture Kits are fast and efficient systems for purifying active tyrosine phosphorylated proteins and interactors in mammalian cell lysates. The assay procedure uses one of several GST-tagged SH2 domain peptides and glutathione agarose resin to capture and pull down the specific binding partners. Each kit contains 600 µg of specific GST-SH2 Domain and sufficient reagents (including negative controls) to perform six pull-down reactions with approximately 500 µg of cell lysate.