DyLight Labeled Highly Cross-Adsorbed Secondary Antibodies |
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 Bright alternatives to Fluorescein, Rhodamine, Alexa Fluor®, CyDye® and LI-COR® Fluorescent Dye conjugates
Thermo Scientific DyLight-Labeled Highly Cross-Adsorbed Secondary Antibodies show minimum cross-reactivity and are suitable for multiplex experiments. They are designed to reduce nonspecific background staining and offer high specificity, low background and increased assay sensitivity. With these conjugates, incubation times are reduced and there is good lot-to-lot consistency.
Each highly cross-adsorbed antibody conjugated to a DyLight Dye was tested for cross-reactivity in plate-based IgG binding assays. They did not exhibit any cross-reaction to human, bovine, horse, rabbit, swine or rat IgGs. The degree of cross-reactivity was determined by ELISA and was typically less than 1% of the desired signal. Our goat anti-mouse conjugates showed low levels of cross-reactivity with rat IgG; however, similar levels of cross-reactivity were observed in other commercially available conjugates. The affinity-purified goat anti-mouse IgG (H+L) was solid-phase adsorbed to minimize cross-reaction with human, bovine, horse, rabbit and swine serum proteins, while the affinity-purified goat anti-rabbit IgG (H+L) was solid-phase adsorbed to minimize crossreaction with human, mouse and rat serum proteins.
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Alexa Fluor is a trademark of Molecular Probes Inc.
CyDye is a trademark of GE Healthcare UK Ltd.
LI-COR is a trademark of LI-COR Inc.
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1 Items Total 995.00 USD