The Thermo Scientific Pierce Fast Western Transfer System reduces protein transfer time from SDS-PAGE to a membrane from 45 minutes to only 10 minutes. The system consists of two parts, the Pierce Fast Semi-Dry Transfer Buffer and the Pierce Fast Semi-Dry Blotter. Use our cost-effective Western Blot Transfer System to obtain membranes that are Western blotting-ready in 10 minutes without the need to purchase special consumables.
About the Pierce Fast Transfer System
The Pierce Fast Semi-Dry Blotter is an Western transfer apparatus that enables rapid transfer of proteins or nucleic acids from a gel to a membrane. When used with the Thermo Scientific Pierce Fast Semi-Dry Transfer Buffer, efficient protein transfer can be achieved in under 10 minutes. The Pierce Fast Semi-Dry Blotter also can be used for conventional semi-dry transfer using traditional buffers and Western blotting protocols. The major advantages of fast semi-dry blotting are reduced buffer consumption, uniform electric field, high transfer efficiency, shorter transfer times and higher throughput (up to 4 mini-gels).
The Pierce Fast Semi-Dry Transfer Buffer is designed to accelerate the transfer of proteins from SDS-PAGE gels to nitrocellulose or PVDF membranes in 7-10 minutes compared with the traditional 45-minute transfer. This semi-dry transfer buffer is compatible with other semi-dry transfer units. The formulation does not require methanol and is supplied as a 10X concentrate that is simply diluted with deionized water before use.
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