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 Biotin-Binding Microplates

  Thermo Scientific Pierce Biotin-Binding Plates are available in High Sensitivity (HS), Standard Binding Capacity (SBC) and High Binding Capacity (HBC) formats to facilitate different assay needs. The High Sensitivity Biotin-Binding Plates are designed specifically for assays requiring the detection of molecules at low concentrations (down to 5 ng/ml). The Standard Binding Capacity Biotin-Binding Plates are useful for standard ELISA conditions with typical sample concentrations and detection range. The High Binding Capacity Biotin-Binding Plates use our patent-pending plate-coating technology to provide a wider detection limit than our standard binding capacity plates. Thermo Scientific Pierce Streptavidin and NeutrAvidin Coated Plates are available in 96-well and 384-well plate formats and pre-blocked. These Pierce Biotin-Binding Plates are available in clear, white* and black plates can be used with colorimetric, chemiluminescent and fluorescent detection methods, respectively. Whether detecting your signal by fluorescent, colorimetric or chemiluminescent methods, these plates make it easy to develop an ELISA for any sample with a low-level target.

Streptavidin and NeutrAvidin Biotin-Binding Proteins are not glycosylated which prevents nonspecific lectin-binding observed with avidin coated plates. Biotin-binding plates are ideal for applications when direct adsorption to polystyrene denatures antibodies or the target molecule. Streptavidin has an isoelectric point of 5-6, resulting in low nonspecific interactions. However, NeutrAvidin Protein has a near-neutral pI (6.3), resulting in even lower nonspecific interactions. NeutrAvidin Protein has the additional advantage of lacking the bacterial RYD sequence found on streptavidin, which eliminates nonspecific binding due to the RGD binding domain of adhesion receptors present in a variety of cells.


  Standard Binding Capacity (SBC) High Binding Capacity (HBC) High Sensitivity (HS)
Application General ELISA screening Detect high concentrations of biotinylated molecules Detect low concentrations of biotinylated molecules
Streptavidin coated plates 31-1,250 ng/ml 62-10,000 ng/ml 5-300 ng/ml
NeutrAvidin coated plates 15-300 ng/ml 15-2,500 ng/ml 5-125 ng/ml




* Thermo Scientific Pierce High Sensitivity Biotin-Binding Plates are currently only available in clear and black 96-well strip plate formats.