shRNA Research Products

Transient, stable, inducible and in vivo RNAi

The Thermo Scientific product portfolio now has the largest offering of shRNA technology provided in either plasmid or viral format.  This includes Thermo Scientific Open Biosystems Products’ microRNA-adapted shRNA (shRNAmir) libraries targeting human and mouse genomes.

Developed in collaboration with Greg Hannon at Cold Spring Harbor Labs and Steve Elledge at Harvard University, shRNAmir triggers for RNAi produce increased specificity and efficient knockdown at single copy.  Lentiviral, inducible lentiviral and retroviral vector formats are available to allow the broadest use in all cell lines including those resistant to transfection such as stem cells, primary cells and neuronal cells.

Additionally, the microRNA-adapted Thermo Scientific Dharmacon SMARTvector® shRNA lentiviral platform combines the advantages of shRNAmir and lentiviral delivery and is provided as ready-to-use high-titer virus for reduced toxicity.

shRNAmir Starter Kits

• Guaranteed knockdown
• NEW! RNAintro Transduction Kit: Five shRNAmir constructs, controls and Lentiviral Packaging System 
• RNAintro Transfection Kit: Five shRNAmir constructs, controls  and Arrest-In™ transfection reagent

Specific and Efficient Knockdown

• Lentiviral shRNAmir
  > pGIPZ - Constitutive
  > pTRIPZ - Inducible
• Ready-to-use lentiviral particles
  > SMARTvector 
• Retroviral shRNAmir with variable promoters
  > pSM2 - U6 promoter
  > pSMP - Viral LTR promoter
• Lentiviral shRNA
  > pLKO.1 - U6 promoter
  

Multiplexed RNAi Screening Library

• Genome-scaled pooled screening library
  > Decode RNAi 

• Defined by Gene Ontology (GO)
• Available as lentiviral or retroviral shRNA
• Custom sets can be created
• Corrosponding cDNAs and ORFs for hit validation or antibodies for detection

• SMARTvector shRNA Lentiviral Particles Product List