2008 US Mass Spectrometry Courses
Finnigan LCQ Operations - 4 Days
The LCQ Operations course is designed for users new to the LCQ and covers mass spectrometry and chromatography in general, with emphasis on small molecule applications. The course involves lecture material, software training and hands-on sessions, involving tuning, calibration and comprehensive LC/MS method development, using both electrospray and atmospheric pressure chemical ionization. A detailed training manual is included with a CD containing all data acquired during the training course and additional material.
The course material includes:
- Ion Trap Theory
- Tuning and Calibration
- Hands-on APCI and ESI MS
- Instrument Method Development for LC/MS
- Multi-stage MSn Method Building
- Quantitative Analysis
- XCalibur Software
- Basic Maintenance
Finnigan LCQ Biotech Operations - 4 Days
This course is intended for users of the LCQ family of instruments using the instrument for peptide and protein work. The course describes: Hands-on activities will involve performing experiments in tuning, data acquisition, and interpretation. Course size is limited to maximize experience with the instrumentation. There are no prerequisites for this course. The course is taught using the current shipping version of the instrument and software and may not reflect the instruments or software you are currently using.
The course material includes:
- Ion Trap Theory
- Tuning and Calibration
- Hands-on ESI and NSI MS
- Instrument Method Development for nano LC/MS
- Data Dependent Method Design
Finnigan LXQ Operations - 4 Days
The LXQ Operations course is designed for users new to the LXQ and covers mass spectrometry and chromatography in general, with emphasis on small molecule applications. The course involves lecture material, software training and long hands-on sessions, involving tuning, calibration and comprehensive LC/MS method development, using both electrospray and atmospheric pressure chemical ionization. A detailed training manual is included with a CD containing all data acquired during the training course and additional material.
The course material includes:
- Ion Trap Theory
- Tuning and Calibration
- Hands-on APCI and ESI MS
- Instrument Method Development for LC/MS
- Multi-stage MSn Method Building
- Quantitative Analysis
- XCalibur Software
- Basic Maintenance
LTQs XL Operations - 4 Day
The LTQ Operations course is designed for users new to the LTQ and covers mass spectrometry and chromatography in general, with emphasis on small molecule applications. The course involves lecture material, software training and long hands-on sessions, involving tuning, calibration and comprehensive LC/MS method development, using both electrospray and atmospheric pressure chemical ionization. A detailed training manual is included with a CD containing all data acquired during the training course and additional material.
The course material includes:
- Ion Trap Theory
- Tuning and Calibration
- Hands-on APCI and ESI MS
- Instrument Method Development for LC/MS
- Multi-stage MSn Method Building
- Quantitative Analysis
- XCalibur Software
- Basic Maintenance
LTQ XL ETD - 4 Days
This course is designed for experienced LC/MS users who perform protein analysis on the LTQ XL mass spectrometer equipped with the electron transfer dissociation (ETD) source. The course will introduce students to the use of ETD as an alternative to collision induced dissociation (CID) for the structural analysis of proteins and peptides and the identification of post-translational modifications (PTM). In addition, the course will cover electrospray ionization (ESI) of protein and peptides, tuning using nanospray, and optimization of Data Dependent TM acquisition. The curriculum will feature lectures, in-depth discussions of qualitative analysis, and hands-on training using the latest versions of Xcalibur TM and BioWorks TM software. A detailed training manual and a CD containing data acquired during the course are included, along with additional course materials.
Course topics include:
- Ion trap theory
- ETD theory
- Tuning and calibration
- Hands-on ESI and NSI MS
- Instrument method development for nano LC/MS
- Data Dependent method design using ETD and CID
- PTM methods
- Xcalibur software for qualitative methods
- BioWorks software
- Basic LTQ XL and ETD maintenance
Note: Students seeking detailed instruction of small molecule analysis qualitative/quantitative data processing should attend the LTQ XL Operations course. Students seeking detailed instructions of protein and peptide analysis who do not have access to ETD in their own laboratories should attend the LTQ XL Biotech Operations course. Students who desire Xcalibur software training should attend the Xcalibur Software Operations course.
Finnigan LTQ Orbitrap Operations - 4 Days
The LTQ-Orbitrap Operations course is designed for users that have either completed the LC/MS for Beginners course or have previous LC/MS experience. Experience in FTMS is not required. The course covers mass spectrometry and chromatography in general with an emphasis on small molecule, accurate mass applications.
The course material includes:
- Ion Trap Theory
- Orbitrap Theory
- Tuning and Calibration
- Hands-on APCI and ESI MS
- Instrument Method Development for LC/FTMS
- Multi-stage MSn Method Building
- Parallel Detection Methods
- Accurate Mass Methods
- XCalibur Software
- Basic LTQ Maintenance
Finnigan LTQ FT Ultra Small Molecule Operations - 4 Days
The LTQ-FT Operations course is designed for users that have either completed the LC/MS for Beginners course or have previous LC/MS experience. Experience in FTMS is not required. The course covers mass spectrometry and chromatography in general with an emphasis on small molecule, accurate mass applications.
Prerequisite: Practical LC/MS for Beginners course or previous experience developing LC/MS methods.
The course material includes:
- Ion Trap Theory
- ICR Theory
- Tuning and Calibration
- Hands-on APCI and ESI MS
- Instrument Method Development for LC/FTMS
- Multi-stage MSn Method Building
- Parallel Detection Methods
- Accurate Mass Methods
- XCalibur Software
- Basic LTQ Maintenance
Finnigan LTQ FT Ultra Biotech Operations - 4 Days
This course is intended for users of the LTQ-FT Hybrid Linear Ion Trap using the instrument for peptide and protein work. The course describes:
Hands-on activities will involve performing experiments in tuning, data acquisition, and interpretation. Course size is limited to maximize experience with the instrumentation. There are no prerequisites for this course.
The course material includes:
- Ion Trap Theory
- FTMS Theory
- Tuning and Calibration
- Hands-on ESI and NSI MS
- Instrument Method Development for LC/FTMS
- Nano-flow LC Method Development
- Data Dependent Method Design
- Post Translational Modification Methods
- Parallel Detection Methods
- Accurate Mass Methods
- XCalibur Software for Qualitative Methods
- Bioworks 3.2 Software
- Basic LTQ Maintenance
Finnigan TSQ Quantum Operations - 4 Days 
This course will familiarize the Finnigan TSQ Quantum Discover Max or Ultra user with basic instrument operation, including API and quadrupole theory, tuning, calibration, data collection, maintenance, and general functionality of the Xcalibur software package.
The course material includes:
- Development of the TSQ Quantum
- Fundamentals of Mass Spectrometry
- Ion Max Source
- TSQ Quantum Hardware
- TSQ Quantum Scan Modes
- TSQ Quantum Instrument Control
- Xcalibur
- XReport
- User Maintenance
Finnigan TSQ Quantum Accurate Mass Operations - 3.5 Days
This course will inculcate the Finnigan TSQ Quantum Ultra AM user with detailed operational procedures requisite for the acquisition of accurate mass data. Nominal/High-resolution/Accurate Mass tuning and calibration, and data collection will be covered. It is encouraged that students have prior exposure to accurate mass procedures, as well as in-depth understanding of the principles of organic chemistry and enhanced chromatographic skills.
The course material includes:
- Introduction to Accurate Mass Measurement
- Important Points for AM Operation
- Instrument Calibration
- Lock Masses
- Examples of AM Data Acquired on the TSQ Quantum
- Data Acquisition through the Tune Page and Xcalibur
- Xcalibur Processing of Accurate Mass Data
Finnigan Surveyor MSQ Plus Operations - 3 Days
The MSQ+ Operations Course is designed to familiarize the new MSQ+ user with basic instrument operation, including API and mass spectrometry theory. The class will also cover compound optimization, calibration of the instrument, data collection, and user level maintenance. The Xcalibur software package will thoroughly be explored. Students will gain hands-on experience and become familiar with the MSQ+ Instrument.
The course material includes:
- LC/MS operational methodology
- Overview of quadrupole instrumentation
- Introduction to MS theory
- API techniques
- Hardware components specific to the MSQ+
- Method development
- Calibration and data acquisition
- Compound Optimization
Finnigan LTQ Orbitrap Biotech Operations - 4 Days
The LTQ-Orbitrap Biotech Operations course is designed for users that have either completed the LC/MS for Beginners course or have previous LC/MS experience. This course is specific to the Finnigan LTQ-Orbitrap Mass Spectrometer product and will include instruction for electrospray ionization (ESI) of proteins and peptides, tuning using nanospray, and optimization of data-dependent acquisition. In addition, there will be an in depth discussion of qualitative analysis and processing accurate mass methods with the XCalibur and BioWorks 3.2 software packages followed by hands-on applications of software to data sets.
The course material includes:
- Ion Trap Theory
- Orbitrap Theory
- Tuning and Calibration
- Hands-on ESI and NSI MS
- Instrument Method Development for LC/FTMS
- Nano-flow LC Method Development
- Data Dependent Method Design
- Post Translational Modification Methods
- Parallel Detection Methods
- Accurate Mass Methods
- XCalibur Software for Qualitative Methods
- Bioworks 3.2 Software
- Basic LTQ Maintenance
BioWorks/SEQUEST Software Module - 2 Days
This course is intended for peptide and protein users of the ion trap and triple quadrupole family of instruments. The course teaches the software package only and assumes the user is versed in data dependent scanning and Xcalibur.
The course material includes:
- The components of Bioworks
- Database searching and interpretation of the results
LTQ/LTQ XL Biotech Operations – 4 Days
This instrument-specific course focuses on electrospray ionization (ESI) of proteins and peptides, tuning using nanospray, and Data Dependent™ acquisition. Additionally, the course provides in-depth discussion and hands-on learning of qualitative analysis using the latest version of Xcalibur™ and BioWorks™ software. A detailed training manual and a CD containing data acquired during the course are included, along with additional course materials.
Course topics include:
- Ion trap theory
- Tuning and calibration
- Hands-on ESI and NSI MS
- Instrument method development for nano LC/MS
- Data Dependent method design
- Post-translational modification methods
- Xcalibur software for qualitative methods
- BioWorks software training
- Basic LTQ maintenance
Prerequisite: Practical LC/MS for Beginners course or previous experience developing LC/MS methods
Accela UHPLC Training - 2 Days
This introductory course is designed for users who are new to the Accela™ UHPLC System. Through lectures and hands-on lab experience, students will gain an understanding of the method development process related to liquid chromatography for standard and high-pressure separations.
The course will include information about:
- UHPLC theory and stationary phase design
- The various modes of chromatographic separation (i.e., Reversed Phase, Normal Phase, etc.)
- Developing robust methods for high-pressure separations
- Mobile phase selection
- Troubleshooting separations
Mass Frontier 5.0 Software Operations Course - 2 Days
The aim of this training course is to provide new users with the ability to use Mass Frontier 4.0 software to its full potential. Detailed presentations will be given on all Mass Frontier modules together with hands on exercises in order to ensure understanding of all the processes. The students will become familiar with the subjects of database generation and manipulation, spectra interpretation, as well as compound classification with the use of statistics. By the end of the course they should be able to apply all software applications for their own purposes.
The 2-day course will provide in-depth presentations and hands-on exercises on all the modules Mass Frontier 4.0 offers: Database Manager, Chromatogram Processor, Fragments and Mechanisms, Spectra Classifier. In addition, a short course on the interpretation of CID spectra will be included in order to allow the users to update the Fragmentation Library module with new mechanisms.
QuickQuan Quantum Operations - Quantitative Analysis - 3 Days
Gain a complete knowledge of QuickQuan- the powerful software package that operates with Xcalibur and your TSQ system. With minimal user input, the software aids drug discovery labs to automatically optimize new chemical entities (NCE’s) for SRM experiments, generate LC/MS/MS methods, and perform quantitative analysis. The data is processed automatically, generating instant customized results using Xreport templates or custom Excel macro templates. (Course also known as TSQ Quantum Quick Quan)
Upon completion, the user will gain a thorough understanding of the following using hands-on software and hardware practicals:
- Operation of quantum instrumentation
- QuickQuan software including EZTune
- Plumbing of autosampler and HPLC systems
- Maintenance and troubleshooting
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