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2008 Proteomics Seminar Tour

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Agendas and Dates:
Mon Oct 6Tours
Tue Oct 7Utrecht
Wed Oct 8Copenhagen
Thu Oct 9Stockholm
Wed Oct 15Manchester
Thu Oct 16Dublin
Fri Oct 17London
Mon Oct 20 Vienna
Tue Oct 21Munich
Wed Oct 22 Basel
Thu Oct 23 Berlin
Mon Nov 10Madrid
Wed Nov 12 Barcelona
Thu Nov 13Milan

Abstracts
• Juan Casado-Vela
Applicability of LTQ-Orbitrap MS to address proteomic studies: high-throughput protein ID, changes of protein level using iTRAQ and de novo sequencing.

• Bruno Domon
Novel Strategies Enabling High-Throughput Proteomic Analyses

• Warwick Dunn
The role of the LTQ-Orbitrap in metabolic profiling of mammalian metabolomes

• Melanie Flint
Molecular determination of stress hormone-mediated drug resistance to Paclitaxel in breast cancer

• David Good
Characterizing the Human Embryonic Stem Cell Proteome ? Life with an ETD-Enabled Orbitrap

• Claus Jorgensen
Mapping of signaling networks in boundary formation by quantitative mass spectrometry and RNAi

• Patrick Kiefer
Metabolome analysis by liquid chromatography high resolution mass spectrometry using the LTQ-Orbitrap

• Bernhard Kuester
Robust iTRAQ peptide quantification on an LTQ-Orbitrap mass spectrometer and its application to chemical proteomics

• Martin Larsen
Phosphoproteomics ? technologies and application to the study of depolarization-dependent protein phosphorylation in nerve terminals

• Matthias Mann
Towards complete proteome quantitation

• Nick Morris
The use of phosphoproteomics to discover novel AMPK substrates

• Scott Peterman
Expediting targeted protein quantitation method development using a triple quadrupole mass spectrometer: software and hardware tools to address hypothesis-based and bioinformatics-based approaches.

• Douglas Phanstiel
Characterizing the Human Embryonic Stem Cell Proteome ? Life with an ETD-Enabled Orbitrap

• Maria Prieto (Spanish)
Análisis de Imagen por Espectrometría de Masas con el MALDI LTQ XL y MALDI LTQ Orbitrap: importancia de MS3 y del rango dinámico

• Sarah Robinson
Expediting targeted protein quantitation method development using a triple quadrupole mass spectrometer: software and hardware tools to address hypothesis-based and bioinformatics-based approaches

• John Rogers
Selective Enrichment and Quantitation of Phosphoproteins and Phosphopeptides Involved in Cell Proliferation

• Vladimir Shulaev
Metabolomics technology and bioinformatics.

• Carsten Sonksen
Full sequence and PTM characterization of recombinant proteins with analytic LC-Orbitrap MS/MS, software tools and what we still need.

• Kerstin Strupat
MALDI Produced Ions Inspected with a Linear Ion Trap - Orbitrap Mass Analyzer

• Peter Verhaert
Mass Spectrometry Imaging of Neuropeptides

• Rob Vreeken
Metabolomics and metabolite profiles for phenotyping of individuals.

• Wolfram Weckwerth
Genome-wide metabolomics, proteomics and data integration: from molecule to organism

• Amy Zumwalt
Early markers of kidney transplant rejection:
Proteomic workflows for discovery and the development of non-invasive, targeted quantitation assays

Proteomics 2008 - Warwick Dunn

The role of the LTQ-Orbitrap in metabolic profiling of mammalian metabolomes

Warwick Dunn1

1Manchester Centre for Integrative Systems Biology, University of Manchester

The investigation of the total complement of endogenous and exogenous metabolites in a biological system (microbial, plant, mammalian) is termed metabolomics. The total collection of thousands of metabolites, termed the metabolome, provide complex systems to study and the detection and identification of all metabolites is currently not feasible. The coupling of sub-2µm high performance liquid chromatography with the LTQ-Orbitrap mass spectrometry system has provided a powerful platform for the detection and identification of 100-1000s of metabolites in a single analytical run. Currently, the Bioanalytical Sciences Group at The University of Manchester employ sub-2µm HPLC coupled to the LTQ-Orbitrap mass spectrometry system as a tool to investigate mammalian metabolomes

In this presentation the advantages of the platform for metabolic profiling of complex metabolomes is discussed including the investigation of pre-eclampsia and heart failure. Also the characterisation of metabolites employing a range of strategies (accurate mass, MS/MS and mass spectral libraries) will also be discussed.