Applicability of LTQ-Orbitrap MS to address proteomic studies: high-throughput protein ID, changes of protein level using iTRAQ and de novo sequencing
Juan Casado-Vela
BioGUNE
Among the plethora of tasks routinely carried out in proteomic labs, we focus on the performance of LTQ Orbitrap XL mass spectrometer for 1) high-throughput protein identification, 2) accuracy for quantification of protein changes using iTRAQ labelling and 3) de novo sequencing of peptides from non-sequenced organisms. We show that the high mass accuracy reached by the Orbitrap detector precludes the interference of nearly-isobaric masses, increases database search speed and minimizes the false discovery rate, thus, facilitating all these tasks.
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