This section includes tips which can help your work with the Multidrops and give insights which are not readily available in the manual. In this section is included tips from our customers who have the greatest experience in their hands. If you have something you would like to share with others, please contact us using contact details provided in the Ask & Share section.
Multidrop Combi, Multidrop 384 and Multidrop DW
Cleaning procedures for dispensing cassettes:
"The dispensing cassette can be hooked up to a faucet that has a piece of tygon tubing attached that is wide enough to accommodate the end of the cassette with the tubing weight on the end. Then the water is just turned on. This eliminates tension due to the pump."
"The whole dispensing cassette can be placed directly into the sonication bath for cleaning."
"The tubings are often coated with the base buffer used for the assay to prevent sticking. For coating, for example, is used the 10 x buffer concentration."
Think also other components involved:
"We were dispensing with the Multidrop Combi into a 384-well plate and had weird discrepancy of results in odd-even rows. We were using the Tecan Safire 2 microplate reader in polarization mode.The Tecan representative suggested that we would introduce a delay of 300 msec in the measurement parameter menu, and that really eliminated the odd-even row discrepancy completely. (A delay of 100 msec worked just as well.) I assume that the problem is in the plate transport in the Safire2 microplate reader, which has differential effects on the liquid meniscus depending on whether the plate transport is towards left or right. But not knowing this, I could have sworn that the problem was due to the unequal filling of the rows by the Multidrop, the difference in the readings without the time delay being about 8%."
Multidrop Combi nL
"We had problems when we evaluated the accuracy values with the Artel MVS dye system. The dye is diluted in DMSO in this method, and once the dye is diluted in DMSO, the final solution is actually only 80% DMSO which is different enough from the 100% DMSO solution to affect the accuracy values. So, 80% DMSO should be used for calibrating the Multidrop Combi nL if this system is utilized."
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