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Protein Modification

Protein modification by reducing agents

TCEP is a potent, water-soluble, odorless reducing agent that is a popular alternative to DTT and mercaptoethanol.

Cross-linking Technical Handbook

This 47-page handbook begins with a basic discussion on crosslinking and the reagents that are used. It also contains a discussion on various applications where crosslinking has been applied, including the powerful label-transfer technique for identifying or confirming protein interactions.

Crosslinking and Protein Modification

Proteins can be easily modified by various methods to alter their structure or properties. Common modifications include crosslinking, fragmenting, denaturing, reducing disulfides, and attaching various prosthetic groups (e.g. PEGylation) to a protein. View more information on Protein Crosslinking and Modification products.

NHS and Sulfo-NHS

N-hydroxysulfosuccinimide (Sulfo-NHS) and its uncharged analog N-hydroxysuccinimide (NHS) are used to convert carboxyl groups to amine-reactive Sulfo-NHS esters.

PEG reagents - Amine Reactive

Attach branched methyl-capped polyethylene oxide (PEO) moieties to amine groups. Increase solubility and stability and reduce immunogenicity of peptides and proteins.