Protein Labeling

Proteins can be covalently modified in many ways to suit the purpose of a particular assay. A wide variety of immunological and other protein methodologies involve the use of labeled antibodies or other proteins. Enzymes, biotin, fluorophores and radioactive isotopes are commonly used in biotechnology applications to provide a detection signal. They can be conjugated to antibodies, avidin, streptavidin, Fc binding proteins such as Protein A or G, or other proteins. The labeled molecule can then be used in a variety of detection systems.

The highly specific interaction of avidin with the small vitamin biotin can be a useful tool in assay systems designed to target and detect biological analytes.

Convenient kits and activated fluorescent dyes for labeling amines, sulfhydryls and other functional groups with FITC, fluorescein, rhodamine or DyLight Fluor alternatives to Cy Dyes and Alexa Fluors.

• Biotinylate carboxyl groups with EDC and Biotin Hydrazide
• Block endogenous biotin
• Calculate dye:protein (F/P) molar ratios
• Preferentially biotinylate N-terminal alpha-amino groups in peptides

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