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  Appliskan


The Appliskan multimode reader offers all detection technologies in one small-sized, compact and robust instrument for both current and future application needs in an ever changing research environment.
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Appliskan is a true multimode microplate reader capable of measuring Luminescence, Absorbance, Fluorescence Intensity, Fluorescence Polarization, and Time-Resolved Fluorescence in 6- to 384-well plates. Additionally, the Appliskan provides possibilities for dual-label measurements, such as BRET, FRET and TR-FRET assays.

Appliskan has the added advantage of onboard shaking and incubation for temperature-sensitive cellular assays. Additionally, the possibility of having up to two dispensers onboard makes the Appliskan an ideal instrument also for kinetic applications. Appliskan provides the flexibility to carry out a wide range of microplate assay types, such as cytotoxicity, DNA analysis, immunoassays, multiplexing and protein activity, amongst many others.

For maximal performance Appliskan is powered by SkanIt Software, which allows easy assay setup, comprehensive data handling and convenient report formatting.
 Appliskan
 Product# Price  Weight  Power Requirements  Size  
           
 5230000      -    27kg 100-240 V, 50/60 Hz 375 mm (W) x 500 mm (D) x 340 mm (H) 14,8 ‘’ (W) x 19,7 ‘’ (D) x 13,4 ‘’ (H)   SELECT  
 5230010      -    28.5 kg 100-240 V, 50/60 Hz 375 mm (W) x 500 mm (D) x 340 mm (H)   SELECT  
 5230020      -    30 kg 100-240 V, 50/60 Hz 375 mm (W) x 500 mm (D) x 340 mm (H)   SELECT  
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Specifications:
Technical specifications :
Plate types :6 to 384-well plates, defined by Thermo in SkanIt Software
Incubator temperature range :From ambient + 4°C to 45°C
Shaking method :Linear shaking
Dispenser(s) :Up to two optional dispensers
Dispensing volume :5 – 500 µl with 1 µl increments
Fluorometry :
Excitation wavelength range :200 – 1000 nm
Emission wavelength range :360 – 820 nm
Sensitivity :Fluorescence intensity: < 2 fmol fluorescein/well, 384-well plate Time-resolved fluorescence: < 20 amol Europium/well, 384-well plate
Precision :Fluorescence polarization: < 10 mP 1 nM fluorescein, 96-well plate
Dynamic range :> 5 decades
Photometry :
Wavelength range :200 – 1000 nm
Linearity :0 – 2.5 Abs (96-well plates) 0 – 2 Abs (384-well plates)
Measurement range :0 – 4 Abs
Absorbance resolution :0.001 Abs
Luminometry :
Emission wavelength range :Standard mode: 360 – 820 nm High-sensitive mode: 300 – 630 nm
Sensitivity :High-sensitive mode: < 10 amol ATP/well, 384-well plate Standard mode: < 200 amol ATP/well, 384-well plate
Dynamic range :> 5 decades