Thermo Scientific DyLight Fluorescent Labeled Phosphoramidites feature absorption and emission spectra suitable for a broad range of applications and can be used in research applications without the constraints of licensing and royalty fees.
Product Detail
Introduce a fluorescent tag at the 5'-position of an oligonucleotide
Easily incorporated into the oligo using standard synthesis and deprotection procedures
Process and Supply Chain Control:
A controlled raw material supply chain and established vendor partnerships ensure consistency and security of supply
Processes are strictly controlled and monitored from material procurement through final QC and product release under ISO9001 standards, providing batch-to-batch reproducibility
All phosphoramidites are supplied with lot-specific quality control data
DY547, DY647 and DY677:
DyLight DY547, DY647 and DY677 phosphoramidites have absorbance and emission spectra comparable to Cy*3, Cy5 and Cy 5.5 respectively
DyLight labeled oligonucleotides are stable from pH 3-9 and have photostability comparable to or exceeding oligos labeled with Cy dyes
Payment of licensing fees is not required for use of DyLight dyes in research applications
FAM-6 and Fluorescein:
FAM-6 and fluorescein labeled oligonucleotides are used in sequencing and PCR applications
These phosphoramidites may be used on all automated DNA synthesizers using standard synthesis procedures
FAM-6 is further purified to remove the 5-isomer and leaves ≥95% of the 6-isomer; the higher purity of the single isomer is ideal in applications requiring higher sensitivity
Flexible Packaging:
Common pack sizes fit most commercially available synthesizers and are in stock for immediate delivery
Custom and bulk packaging, including multikilogram lots, are available