Thermo Scientific Modifying Phosphoramidites are for the addition of a primary amine to the 5' position of an oligonucleotide or to incorporate an amine within the sequence.
Product Detail
Process and Supply Chain Control:
A controlled raw material supply chain and established vendor partnerships ensure consistency and security of supply
Processes are strictly controlled and monitored from material procurement through final QC and product release under ISO9001 standards, providing batch-to-batch reproducibility
All modifiers are supplied with lot-specific quality control data including HPLC and phosphorous NMR analyses
Amino Modifiers:
The amino modification provides a site for attachment of fluorescent labels such as biotin or NuLight* esters
Oligos with an amino modification can also be attached to surfaces in DNA chip applications
The 5' amino modifier amidites are supplied with MMT or TFA protection
The MMT modifier allows for trityl-on purification of the resulting oligonucleotide
The TFA group is removed during ammonia deprotection
Phosphorylation:
The phosphorylating phosphoramidte is for the addition of a phosphate to the 5' or 3' position of the oligonucleotide during standard synthesis protocols
Flexible Packaging:
Common pack sizes fit most commercially available synthesizers and are in stock for immediate delivery
Custom and bulk packaging, including multikilogram lots, are available
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