Sera-Mag Magnetic Oligo(dT) particles are mainly used for mRNA isolation and can also be used as a universal base particle for coupling unique oligo sequences. These nominal 1 micron, super-paramagnetic particles are uniform in size with covalently bound oligo(dT) 14. Sera-Mag particles combines the advantages of high surface area, high affinity and high specific activity.
Sera-Mag Magnetic Oligo(dT) particles are colloidally stable in the absence of a magnetic field, however the particles can be separated rapidly and completely from suspension when a magnetic field is applied. Binding of polyadenylated RNA (poly-A+ RNA) to the covalently bound oligo(dT) groups on the surface is easily accomplished using standard hybridization conditions. Other RNA species (rRNA and tRNA) do not contain poly A+ and therefore will not bind to the oligo(dT) beads. The isolated mRNA can be used directly in cloning and expression analysis applications.
In addition, the oligo(dT) beads can be used a universal base particle on which to attach your unique oligo sequence. By synthesizing a poly-A tail on your special oligo, you can easily bind this to the dT surface of the particles to create a uniquely coated magnetic particle. For more features and benefits, see the Oligo (dT) technical notes file on the left.
Applications for Sera-Mag Magnetic Oligo(dT) particles include mRNA isolation, cDNA library construction, subtractive hybridization, affinity purification, RT-PCR applications, primer extension and cDNA Microarrays.
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