ReddyMix PCR Buffer & Master Mixes
 
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  ReddyMix PCR Buffer & Master Mixes


Save time post-PCR with our ReddyMix PCR Buffer and Master Mixes. ReddyMix is an all-in-one PCR solution that allows direct loading of PCR products on to agarose gels.
   Product Detail


Applications

  • Routine PCR using genomic, viral and plasmid templates
  • Amplifying fragments up to 12kb
  • RT-PCR

Description

The unique ReddyMix PCR buffer has an inert red tracking dye and gel loading precipitant already added. After thermal cycling, a sample of the reaction can be removed and loaded directly onto an agarose gel without the addition of loading dye. Combining the components for PCR with gel-loading buffer in a single master mix eliminates many pre- and post- PCR set-up steps. ReddyMix Kits give the same high performance as standard ThermoPrime™ Taq DNA Polymerase Kits.

The inert red dye migrates between bromophenol blue and xylene cyanol, at approximately 300bp, depending on the agarose concentration. Since the additives are in the reaction buffer, rather than in the enzyme preparation, the red dye and precipitant are present at optimal concentrations in the final mix.

Features

  • Gel-ready samples. ReddyMix buffers contain a red dye and precipitant, eliminating the need to add gel loading buffer to samples prior to agarose gel electrophoresis.
  • Easy-to-see mix. The inert red dye allows for easy sample visualization during reaction set-up.
  • High thermal stability. Proprietary buffer formulation protects ThermoPrime Taq during high temperature steps, ensuring optimal performance throughout the PCR reaction.
  • Full-length amplification. ThermoPrime Taq is proven to amplify standard templates in excess of 12kb.
  • Convenient storage. Kits can be stored at 4°C eliminating the need for repeated freezing and thawing of individual components.
  • Simple protocol. Master Mix formats contain all the components required for PCR (with the exception of template and primers), reducing protocol steps and minimizing the risk of pipetting errors and sample contamination.
  • Reproducible results. ThermoPrime Taq is fully licensed for PCR, and all mixes undergo extensive quality control testing to ensure batch-to-batch consistency.

Kit  Components

ReddyMix Core Kit – AB-0790


• Vial of ThermoPrime Taq DNA Polymerase (5U/μl)
• Vial of 10X ReddyMix PCR Buffer
• Vial of 25mM MgCl2

ReddyMix Core Kit – AB-0785

• Vial of ThermoPrime Taq DNA Polymerase (5U/μl)
• Vial of 10X ReddyMix PCR Buffer containing 15mM MgCl2

ReddyMix Master Mix – AB-0575

• Vial of ReddyMix PCR Master Mix containing ThermoPrime Taq DNA Polymerase, dNTPs, ReddyMix Buffer, and MgCl2*

* MgCl2 final reaction concentration = 1.5mM
 ReddyMix PCR Buffer & Master Mixes
 Product# Price  Quick Description  
       
 AB-0790/A      -    ThermoPrime (250U) with 10X buffer & separate vial of 25mM MgCl2 (1.25ml)   SELECT  
 AB-0790/B      -    ThermoPrime (2500U) with 10X buffer & separate vial of 25mM MgCl2 2X ReddyMix PCR Master Mix (1000U) containing 1.5mM MgCl2 (12.5ml)   SELECT  
 AB-0785/A      -    ThermoPrime (250U) with 10X ReddyMix PCR buffer containing 15mM MgCl2 (1.25ml)   SELECT  
 AB-0785/B      -    ThermoPrime (2500U) with 10X ReddyMix PCR buffer containing 15mM MgCl2 (12.5ml)   SELECT  
 AB-0575/LD/A      -    1.1X ReddyMix PCR Master Mix (100U) containing 1.5mM MgCl2   SELECT  
 AB-0575/LD/B      -    ThermoPrime (2500U) with 10X ReddyMix PCR buffer containing 15mM MgCl2 (1.25ml)   SELECT  
 AB-0575/DC/LD/A      -    2X ReddyMix PCR Master Mix (100U) containing 1.5mM MgCl2 (1.0ml)   SELECT  
 AB-0575/DC/LD/B      -    2X ReddyMix PCR Master Mix (1000U) containing 1.5mM MgCl2 (10ml)   SELECT  
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Specifications:
Storage :Keep at -20°C for long term storage. Stable at +4°C for up to 4 weeks.
Handling :Avoid repeated freeze-thaw cycles.