Thermo Scientific Thermo-Start Taq DNA Polymerase is a chemically modified hot-start version of our patented ThermoPrime Taq. This hot-start enzyme, combined with an optimized buffer system, gives superior specificity and sensitivity for unparalleled PCR results.
Product Detail
Applications
Hot-start amplification of DNA from complex genomic, viral and plasmid templates
Amplifying fragments up to 4kb
RT-PCR
Description
Thermo-Start Taq DNA Polymerase is engineered to provide maximum sensitivity and specificity for PCR. The chemically-modified Thermo-Start Taq remains completely inactive until it is ‘switched-on’ by a high temperature incubation step at the start of the PCR reaction. This prevents non-specific amplification during reaction set-up, eliminating smearing and the formation of non-target bands.
To protect the enzyme during this 95°C/15 minute activation step, a proprietary buffer is supplied with the enzyme. This ensures an optimal response from the enzyme once it has been activated, resulting in higher yields than those obtained with systems based on chemical modifications alone. When other enzymes have completely lost all activity due to high denaturation temperatures Thermo-Start is still at least 50% active. This thermal stability allows for the high temperature denaturation steps used when working with complex targets.
The combination of Thermo-Start Taq and optimized buffers increases sensitivity and specificity without any compromise on yield. The hot-start system also increases PCR efficiency by preventing amplification of primer dimers formed at low temperatures, preserving reaction components for template amplification.
A High Performance Buffer formulation is available to increase yield while still maintaining a highly sensitive and specific reaction. The High Performance Buffer works extremely well with complex and GC-rich templates.
For direct gel loading kits, see Thermo-Start Taq Reddymix Kits and Master Mixes. ReddyMix buffers contain a red dye and precipitant, eliminating the need to add gel loading buffer to samples prior to agarose gel electrophoresis.
Features
Maximum specificity. Chemically-modified Thermo-Start Taq remains completely inactive until a 95ºC activation step, preventing non-specific amplification.
High thermal stability. Buffer formulation protects Thermo-Start during high temperature steps for efficient amplification throughout cycling, ensuring optimum yield.
Increased efficiency. The use of Thermo-start Taq prevents amplification of primer dimers formed at low temperatures, preserving reaction components for template amplification.
Maximum sensitivity. Thermo-Start Taq delivers efficient, specific amplification of even low copy number targets, making it ideal for applications such as single cell PCR.
Advanced buffer systems. Available with a High Performance buffer for amplification of GC-rich sequences and other challenging targets.
Convenient storage. Kits can be stored at 4°C eliminating the need for repeated freezing and thawing of individual components.
Room temp set-up. Reactions can be set-up at room temperature without the risk of non-specific amplification.
Complete kits
Simple protocol. Master Mix formats contain all the components required for PCR (with the exception of template and primers), reducing protocol steps and minimizing the risk of pipetting errors and sample contamination.
Reproducible results. Thermo-Start Taq is fully licensed for PCR, and all mixes undergo extensive quality control testing to ensure batch-to-batch consistency.
Available Formats
Core Kit with Separate MgCl2 – AB-0908
Vial of Thermo-Start Taq DNA Polymerase (5U/μl)
Vial of 10X PCR Buffer
Vial of 25mM MgCl2
To add Vial of 20mM dNTPs, add ‘N’ to end of part number
Core Kit – AB-1908
Vial of Thermo-Start Taq DNA Polymerase (5U/μl)
Vial of 10X PCR Buffer containing 15mM MgCl2
To add Vial of 20mM dNTPs, add ‘N’ to end of part number
Core Kit with High Performance Buffer – AB-1057
Vial of Thermo-Start Taq DNA Polymerase (5U/μl)
Vial of 10X High Performance PCR Buffer
Vial of 25mM MgCl2
To add Vial of 20mM dNTPs, add ‘N’ to end of part number
Master Mix – AB-0938
Vial of PCR Master Mix containing Thermo-Start Taq DNA Polymerase, dNTPs, PCR Buffer, and MgCl2*
Master Mix with High Performance Buffer – AB-1147
Vial of PCR Master Mix containing Thermo-Start Taq DNA Polymerase, dNTPs, High Performance PCR Buffer, and MgCl2*
3 Artikel Summe 4,660.00 USD 
