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  Red Hot Taq DNA Polymerase



Thermo Scientific Red Hot Taq DNA Polymerase is a robust enzyme mix that delivers high reaction sensitivity and reliable PCR results. The enzyme preparation incorporates an inert red dye that allows for quick, visual verification of enzyme addition during PCR set-up.


   Product Detail


Applications

  • Routine PCR using genomic, viral and plasmid templates
  • Amplifying fragments up to 12kb
  • RT-PCR

Description

Red Hot Taq DNA Polymerase consists of our high performance ThermoPrime Taq DNA Polymerase, plus a unique red indicator dye. This red dye facilitates accurate low volume pipetting and quick enzyme addition verification. Once Red Hot Taq is added to the PCR reaction, the reaction mixture becomes pink, allowing for easy visual verification that enzyme has been added. This inert red dye has no adverse effect on the outcome of PCR (yields are the same as with standard ThermoPrime Taq) and does not interfere with any downstream applications.

Features

  • Licensed for PCR.
  • High performance. Contains ThermoPrime Taq DNA Polymerase to deliver high sensitivity and reproducible results.
  • Easy-to-see. Red dye aids in low volume pipetting and turns PCR reaction mixture pink, allowing for easy visual verification of enzyme addition.
  • Convenient storage. Kits can be stored at 4°C eliminating the need for repeated freezing and thawing of individual components

Available Formats

Enzyme Kit – AB-0406*

  • Vial of Red Hot Taq DNA Polymerase (5U/μl)
  • Vial of 10X PCR Buffer
  • Vial of 25mM MgCl2
  • To add Vial of 20mM dNTPs, add ‘N’ to end of part number

Enzyme Kit – AB-1406*

  • Vial of Red Hot Taq DNA Polymerase (5U/μl)
  • Vial of 10X PCR Buffer containing 15mM MgCl2
  • To add Vial of 20mM dNTPs, add ‘N’ to end of part number

* Please refer to product datasheet for detailed kit content information.

 Red Hot Taq DNA Polymerase
 Product# Price  Description  
       
 AB-0406/A      -    Red Hot Taq DNA Polymerase (100U) with 10X buffer (1.25ml), separate vial of 25mM MgCl2 (1.25ml)   SELECT  
 AB-0406/A/N      -    Red Hot Taq DNA Polymerase (100U), 10X PCR buffer, 20mM dNTPs, 25mM MgCl2   SELECT  
 AB-0406/B      -    Red Hot Taq DNA Polymerase (500U) with 10X buffer (1.25ml), separate vial of 25mM MgCl2 (1.25ml)   SELECT  
 AB-0406/B/N      -    Red Hot Taq DNA Polymerase (500U), 10X PCR buffer, 20mM dNTPs, 25mM MgCl2   SELECT  
 AB-1406/A      -    Red Hot Taq DNA Polymerase (100U) with 10X buffer containing 15mM MgCl2 (1.25ml)   SELECT  
 AB-1406/A/N      -    Red Hot Taq DNA Polymerase (100U), 10X PCR buffer containing 15mM MgCl2, 20mM dNTPs   SELECT  
 AB-1406/B      -    Red Hot Taq DNA Polymerase (500U) with 10X buffer containing 15mM MgCl2 (1.25ml)   SELECT  
 AB-1406/B/N      -    Red Hot Taq DNA Polymerase (500U), 10X PCR buffer containing 15mM MgCl2, 20mM dNTPs   SELECT  
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Specifications:
Storage : Keep at -20°C for long-term storage. Stable at +4°C for up to 4 weeks.
Handling :Avoid repeated freeze-thaw cycles.