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  Taq DNA Polymerase


Thermo Scientific Taq DNA Polymerase is an ultra-pure recombinant Taq polymerase that is able to amplify DNA fragments up to 6kb. Stringent quality controls make this a highly reliable Taq DNA Polymerase for use in standard PCR applications.

   Product Detail


Applications

  • Routine PCR using genomic, viral and plasmid templates
  • Amplifying fragments up to 6kb
  • RT-PCR

Description

Thermo Scientific Taq DNA Polymerase is a 94kDa ultra-pure recombinant thermo-stable DNA polymerase obtained by high level expression of the Taq DNA polymerase gene in Escherichia coli. This enzyme has 5' to 3' polymerase activity and 3' to 5' strand displacement (exonuclease) activity. It also has 3' adenylation activity. Thermo Scientific Taq DNA Polymerase is licensed and optimized for PCR and is able to amplify DNA fragments up to 6kb.

Features

  • Licensed for PCR.
  • Reproducible results. Thermo Scientific Taq is fully licensed for PCR, and all mixes undergo extensive quality control testing to ensure batch-to-batch consistency.
  • Convenient storage. Kits can be stored at 4°C eliminating the need for repeated freezing and thawing of individual components.

Available Formats

Enzyme Kit with Separate MgCl2 – AB-0192*

  • Vial of Thermo Scientific Taq DNA Polymerase (5U/μl)
  • Vial of 10X PCR Buffer
  • Vial of 25mM MgCl2
  • To add Vial of 20mM dNTPs, add ‘N’ to end of part number

Enzyme Kit – AB-1192*

  • Vial of Thermo Scientific Taq DNA Polymerase (5U/μl)
  • Vial of 10X PCR Buffer containing 15mM MgCl2
  • To add Vial of 20mM dNTPs, add ‘N’ to end of part number
     
    * Please refer to product datasheet for detailed kit content information.
 Taq DNA Polymerase
 Product# Price  Description  
       
 AB-0192/A      -    Taq DNA Polymerase (250U) with 10X buffer (1.25ml), separate vial of 25mM MgCl2 (1.25ml)   SELECT  
 AB-0192/A/N      -    Taq DNA Polymerase (250U), 10X PCR buffer, 20mM dNTPs, 25mM MgCl2   SELECT  
 AB-0192/B      -    Taq DNA Polymerase Plus (2500U) with 10X buffer (1.25ml), separate vial of 25mM MgCl (1.25ml)   SELECT  
 AB-0192/B/N      -    Taq DNA Polymerase (2500U), 10X PCR buffer, 20mM dNTPs, 25mM MgCl2   SELECT  
 AB-1192/A      -    Taq DNA Polymerase (250U) with 10X buffer containing 15mM MgCl2 (1.25ml)   SELECT  
 AB-1192/A/N      -    Taq DNA Polymerase (250U), 10X PCR buffer containing 15mM MgCl2, 20mM dNTPs   SELECT  
 AB-1192/B      -    Taq DNA Polymerase (2500U) with 10X buffer containing 15mM MgCl2 (1.25ml)   SELECT  
 AB-1192/B/N      -    Taq DNA Polymerase (2500U), 10X PCR buffer containing 15mM MgCl2, 20mM dNTPs   SELECT  
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Specifications:
Storage : Keep at -20°C for long-term storage. Stable at +4°C for up to 4 weeks.
Handling :Avoid repeated freeze-thaw cycles.