Verso 1-Step RT-PCR Kits

Verso 1-step kits provide a sensitive and convenient method for performing RT-PCR in a single reaction tube. The buffer system of the Verso 1-Step RT-PCR Kit has been specifically optimized to work across a wide range of RNA templates for both transcription and amplification. Special enhancers and additives have been used to generate a reaction mixture which allows both reverse transcriptase and DNA polymerase to work optimally.

The Verso RT Enzyme can be used at temperatures up to 57°C, avoiding problems caused by secondary structure in the RNA template. It also shows a better dynamic range than AMV or MMuLV. The Verso 1-Step RT-PCR Kit can detect as little as 10pg of total RNA or 1pg of mRNA. This allows for accurate detection and quantification of target RNA over a broad template range, ensuring that increases in target RNA concentration give rise to corresponding increases in PCR product.

The master mix format minimizes reagent-handling steps, thus reducing errors and the risk of contamination. It is supplied at a 2X concentration, providing extra flexibility for primer and template concentrations. The Verso RT Enzyme and RNase inhibitor are provided in a separate tube, allowing negative controls without RT enzyme to be carried out easily.

Features

• High yield cDNA. Verso 1-Step Kits contain the highly-efficient Verso RT enzyme with an optimized buffer designed to produce high yield, full length cDNA from even low copy number templates.
• Optimized buffer. Verso 1-Step Kits are based on a balanced buffer to maximize performance of both the reverse transcriptase and DNA polymerase enzymes.
• Unique RT Enhancer. All kits contain RT Enhancer, a unique enzyme that degrades any contaminating genomic DNA during the RT step. It eliminates the need for a DNase I treatment of extracted RNA, saving time and effort.
• Simple protocol. The 1-step reaction and master mix formulations reduce protocol steps and minimize the risk of pipetting errors and sample contamination.
• Reproducible results. All mixes undergo extensive quality control testing to ensure batch-to-batch consistency.

Kit Options

Verso 1-Step RT-PCR Kit

The Verso 1-Step RT-PCR Kit combines the proprietary Verso RT Enzyme and ThermoPrime Taq DNA Polymerase to provide a highly sensitive, easy-to-use system for the detection and analysis of RNA.

Kit Contents

• Verso Enzyme Mix, including RNase Inhibitor
• Verso 1-Step RT-PCR Master Mix (2X)
• RT Enhancer

Verso 1-Step RT-PCR Kit with ReddyMix™

The Verso 1-Step kit is available in ReddyMix format, where the master mix contains an inert red tracking dye and gel loading precipitant. After thermal cycling, a sample of the reaction can be removed and loaded directly onto an agarose gel without the addition of loading dye. Combining the components for PCR and the gel-loading buffer in a single master mix eliminates many pre- and post- PCR set-up steps. ReddyMix Kits give the same high performance as standard Verso 1-Step Kits. The inert red dye also allows for easy sample visualization during reaction set-up.

Kit Contents

• Verso Enzyme Mix, including RNase Inhibitor
• Verso 1-Step RT-PCR ReddyMix Master Mix (2X)
• RT Enhancer

Verso 1-Step Hot-Start RT-PCR Kit

The Verso Hot-Start Kit combines the proprietary Verso RT Enzyme and Thermo-Start Taq DNA Polymerase to provide maximum sensitivity and specificity. The chemically-modified Thermo-Start Taq remains completely inactive until a 95ºC incubation step at the beginning of the PCR reaction. This prevents non-specific amplification during the reaction set-up and the RT step, eliminating non-target bands.

The combination of the Verso RT enzyme, Thermo-Start Taq and optimized buffers increases sensitivity and specificity without any compromise on yield. The hot-start system also increases PCR efficiency by preventing amplification of primer dimers formed at low temperatures, preserving reaction components for template amplification.

The proprietary buffer formulation protects Thermo-Start Taq during high temperature steps, ensuring optimal performance throughout the PCR reaction. This thermal stability allows for the high temperature denaturation steps used when working with complex targets.

Kit Contents

• Verso Enzyme Mix, including RNase Inhibitor
• Verso 1-Step RT-PCR Master Mix (2X), including Thermo-Start Taq
• RT Enhancer