Alternative selectivity for aromatic and moderately polar analytes
Pi-pi interactions with aromatics
Moderate hydrophobicity
Outstanding peak shape and sensitivity
Product Detail
Hypersil GOLD™ phenyl reversed phase columns exhibit alternative selectivity to alkyl chain columns, particularly for aromatic and moderately polar analytes. The Hypersil GOLD phenyl bonded phase contains a butyl linker which allows for superior alignment of the phenyl ring with aromatic molecules, enhancing pi-pi interactions and therefore retention. The C4 linker also provides the stationary phase with moderate hydrophobicity, making it ideal for the separation of analyte mixtures with varying polarity and aromaticity.
Because Hypersil GOLD phenyl columns are based upon the high purity Hypersil GOLD silica, outstanding peak shape and sensitivity is assured. Hypersil GOLD phenyl columns are available with particle sizes from 1.9 to 5 μm.
Antibacterials Separated on Hypersil GOLD Phenyl Column
Mobile phase: A = 20mM KH2PO4, pH 2.5
B = MeCN
Gradient: 20 – 50% B in 15mins
Flow rate: 1mL/min
Temperature: 30°C
Injection Volume: 5µL
Detection: UV @ 225nm
Analyte: 1. Carbadox
2. Thiamphenicol
3. Furazolidone
4. Oxolinic Acid
5. Sulfadimethoxine
6. Sulfaquinoxaline
7. Nalidixic Acid
8. Piromidic Acid