Thermo Scientific HyClone AdvanceSTEM Serum Replacement is designed to maintain murine embryonic stem cells (mESC) in culture.
Product Detail
Providing optimal culture conditions can be one of the greatest challenges in stem cell research, particularly in keeping cells in an undifferentiated state or directing differentiation when desired. HyClone AdvanceSTEM line of stem cell culture products can help you meet those challenges. Classic culture conditions for 129-derived murine embryonic stem cells (mESCs) include culturing in medium containing DMEM, FBS, LIF and co-culturing with primary mouse embryonic fibroblasts (MEFs). Pluripotent mESCs have a very distinct morphology when cultured under these conditions; growing as tightly clustered colonies with smooth phase bright borders. When co-cultured in ES cell media with a mouse embryonic fibroblast (MEF) feeder layer, these stem cell cultures are suitable for electroporation, expansion, or further experimentation.
No requirement for fetal bovine serum
Does not contain serum
Does not contain leukemia inhibitory factory (LIF); supplementation with LIF is required for mESC
Concentrations of 15 to 20% serum
Not recommended for plating mouse embryonic feeder cells (MEFs)
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