Verbatim High Fidelity DNA polymerase Kits

Description

Demanding applications, such as cloning and mutagenesis, require a high performance proofreading enzyme that delivers maximum accuracy and yield. Standard proofreading enzymes such as Pfu or KOD have only moderate fidelity and exhibit reduced processivity due to their proofreading activity. This results in a low yield PCR product that contains an increased number of sequence errors.

Thermo Scientific Verbatim High-Fidelity DNA Polymerase is an advanced proofreading enzyme that delivers superior fidelity and processivity. The Verbatim enzyme is a modified DNA polymerase with enhanced proofreading activity and increased DNA binding affinity. The enhanced proofreading activity generates highly accurate PCR products, and the increased affinity dramatically improves processivity and yield. Additionally, the increased processivity allows for up to 75% reduction in PCR protocol times for faster results.

The increased DNA binding affinity helps Verbatim work through difficult templates, making it a robust enzyme successful on a wide variety of targets. Verbatim High-Fidelity DNA Polymerase is provided with two optimized buffering systems, our high-fidelity buffer for most templates, and a GC buffer for GC-rich and other difficult to amplify templates.

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Features

• Industry Leading Fidelity
• Enhanced Processivity (High Speed of Amplification)
• Two buffering systems for success with a broad range of templates
• High Fidelity buffer for general fragment amplification
• GC Buffer for high-GC and difficult-to-amplify targets

Benefits

• Accurate amplification with high product yield for success in downstream applications.
• Broad Template Flexibility - flexible buffering system for optimal results with both standard and problematic targets
• High speed of amplification - potential to reduce protocol times by up to 75%

Applications

• Enzyme of Choice for Cloning
• Mutational Analysis
• Sequencing
• Long Range PCR Capabilities

Technical Information

• The enhanced DNA binding affinity increases processivity to deliver high yields even with short extension times.
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• Shortened extension times allow for up to 75% reduction on overall protocol times.
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• MgCl₂ Concentrations: Both the High Fidelity and GC Buffers are premixed with MgCl₂ at 2.0mM concentration in the final reaction. A separate MgCl₂ vial (supplied) can be used for further MgCl₂ optimization requirements
• Generating A Overhangs for T/A Cloning: Proof-reading polymerases remove 3' A-overhangs necessary for some cloning applications. Many protocols are widely available for the addition of 3' A overhangs post amplification using standard Taq polymerase if required. Please contact our technical support team for more information