The study of protein interactions is vital to understanding how proteins function within cells. Listed here are products to identify and characterize protein interactions by Co-IP, pull-down, far-Western blotting, crosslinking and label transfer.
Product Detail
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Co-Immunoprecipitation and Pull-Down Kits
Convenient kits to affinity purify protein interaction binding complexes via co-immunoprecipitation (co-IP) or non-antibody affinity system (Pull-Down).
DiscoverLight Human Tissue Lysates and Array Kit
Normal and tumor tissue lysate pairs for use in blotting and other screening applications. The Array Kit includes a nitrocellulose membrane grid pre-spotted with lysate pairs, plus chemiluminescent detection reagents.
Far-Western Blotting Kits
Evaluate protein interactions by probing in-gel or on-membrane with biotin-labeled or tagged binding proteins which can be detected secondarily with an HRP-conjugate and sensitive substrate system.
FeBABE Protein Interaction Mapping Kit
The Protein Interaction Mapping Kit contains the FeBABE protein cutting reagent, very useful in mapping interaction sites of known proteins.
Label Transfer Reagents
Biotin-labeled or iodinatable heterobifunctional, cleavable crosslinkers that can be used to tag a bait protein and then transfer the tag onto an interacting protein following a binding interaction.
Photoreactive Amino Acids
Photo-Leucine and Photo-Methionine are amino acid analogs that can be incorporated into proteins in actively growing cells. In vivo protein interactions are covalently crosslinked upon UV-light activation of the diazirine moiety.
UV Lamps for Crosslinker Photoactivation
Compact, 8-Watt ultraviolet lamp for activating aryl azide and diazirine groups in certain photo-reactive reagents to initiate crosslinking between interacting proteins. Long, midrange, and short wavelength settings allow use with a many techniques.
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