The study of protein interactions is vital to understanding how proteins function within cells. Listed here are products to identify and characterize protein interactions by Co-IP, pull-down, far-Western blotting, crosslinking and label transfer.
Product Detail
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Co-Immunoprecipitation and Pull-Down Kits
Convenient kits to affinity purify protein interaction binding complexes via co-immunoprecipitation (co-IP) or non-antibody affinity system (Pull-Down).
Far-Western Blotting Kits
Evaluate protein interactions by probing in-gel or on-membrane with biotin-labeled or tagged binding proteins which can be detected secondarily with an HRP-conjugate and sensitive substrate system.
FeBABE Protein Interaction Mapping Kit
The Protein Interaction Mapping Kit contains the FeBABE protein cutting reagent, very useful in mapping interaction sites of known proteins.
Label Transfer Reagents
Biotin-labeled or iodinatable heterobifunctional, cleavable crosslinkers that can be used to tag a bait protein and then transfer the tag onto an interacting protein following a binding interaction.
Photoreactive Amino Acids
Photo-Leucine and Photo-Methionine are amino acid analogs that can be incorporated into proteins in actively growing cells. In vivo protein interactions are covalently crosslinked upon UV-light activation of the diazirine moiety.
Pierce Magnetic Beads
High-performance magnetic particles and kits with a variety of affinity ligands, including Protein A, Protein G, GST and titanium dioxide (TiO2); optimized for high throughput workflows and automated solutions with KingFisher Flex Instruments.
Protein A Agarose Beads Protein A covalently coupled to agarose has been extensively used as an affinity support for the isolation of a wide variety of IgG molecules from several species of mammals.
Protein A/G Agarose Beads
Protein A/G combines the IgG binding profiles of Protein A and G. The secreted Protein A/G contains four binding domains from Protein A and two from Protein G, resulting in a high-capacity, high-affinity support that binds to a wide array of antibodies.
Protein G Agarose Beads
Protein G is a recombinant protein that binds to the Fc region of many antibodies, but lacks albumin and cell-surface binding sites found in native protein G. Protein G generally has a higher affinity and binds to a broader range of IgG than Protein A.
Protein L Agarose Beads
Great for purifying ScFv or Fab fragments and monoclonal antibodies containing kappa light chains. Thermo Scientific Pierce Protein L Resins are effective for affinity purification of IgG clones that contain specific kappa light chains.
Sulfo-SBED
Label Transfer is a powerful in vitro method for protein interaction discovery. A growing number of publications feature the use of Sulfo-SBED Biotin Label Transfer Reagent to identify previously unknown protein interaction binding partners and to more fully characterize the specific protein binding domains of other protein interactions.
UV Lamps for Crosslinker Photoactivation
Compact, 8-Watt ultraviolet lamp for activating aryl azide and diazirine groups in certain photo-reactive reagents to initiate crosslinking between interacting proteins. Long, midrange, and short wavelength settings allow use with a many techniques.