Cell lysis is the first step in cell fractionation and protein purification. Many techniques are available for the disruption of cells, including physical and detergent-based methods. Thermo Scientific Pierce offers a wide variety of detergent based cell lysis solution for protein extraction as well as protease inhibitors, protein refolding kits and protein stabilizers.
Product Detail
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Detergents
Thermo Scientific Pierce provides several high-purity, low peroxide-containing detergents in clear ampules, sealed under nitrogen.
Extracti-Gel D Detergent Removing Gel
Versatile affinity matrix for the extraction of unwanted detergent from solutions containing proteins or other macromolecules.
Mem-PER Eukaryotic Membrane Protein Extraction Kit
An excellent alternative to gradient methods or ultracentrifugation. The Mem-PER Eukaryotic Membrane Protein Extraction Reagent Kit is for the enrichment of integral membrane proteins from cultured mammalian or yeast cells or from mammalian tissue using a mild detergent-based protocol.
M-PER Mammalian Protein Extraction Reagent
An efficient, ready-to-use mammalian cell lysis solution that eliminates scraping, freeze-thaw and sonication methods. Mitochondria Isolation Kit for Cultured Cells
Only 40 minutes to isolate intact mitochondria with maximum yield. This kit uses a non-mechanical, reagent-based method that allows multiple samples (six) to be processed concurrently.
Organelle Isolation
Optimized kits for subcellular fractionation and isolation of organelles such as nuclei, mitochondria, peroxisomes and lysosomes.
Poppers Cell Lysis Solutions
Poppers Cell Lysis Solutions are a complete line of ready-to-use cell lysis reagents, kits and related products that make cell lysis much easier and faster than traditional methods such as freeze-thaw cycles, sonicators and glass beads.
Protein Stabilizing Cocktail
The Protein Stabilizing Cocktail from Thermo Scientific Pierce is a versatile stabilizing solution that increases the shelf-life of purified or partially purified proteins during routine storage.
SDS-Out SDS Precipitation Reagent
Convenient removal of SDS from microliter volume samples. Works well with SDS concentrations from 0.1-1 percent. Minimal dilution of small-volume samples. Protein sample recovery in 90-100 percent range.
Slide-A-Lyzer Dialysis Cassettes
These cassettes are a convenient means for dialyzing sample volumes from 100 µl to 30 ml. Low molecular weight contaminant removal, buffer exchange, desalting and concentration can be accomplished with this device. The cassette's patented design, which provides a maximum surface area/sample volume ratio, allows for excellent sample recoveries.
T-PER Tissue Protein Extraction Reagent
An extra-mild tissue cell lysis solution designed for total protein extraction from tissue samples.
Zeba Spin Desalting Columns
Desalt sample volumes ranging from 2 µl to 4 ml with Zeba Desalting Columns and experience exceptional protein recovery quickly.
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