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Undyed Reagent Microspheres
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  Undyed Reagent Microspheres


These beads are available in many sizes with a variety of surface chemistries to accommodate a wide range of coupling strategies.

   Product Detail


These microspheres were developed with surfaces designed for attaching biological molecules. A variety of sizes and surface functionalities are available providing researchers with a wide choice of products for applications in diagnostics, genomics, cell labeling/sorting, and other functions. Most of the microspheres are prepared without surfactants and have surfaces optimized for either hydrophobic adsorption or covalent attachment of biological molecules.

Descriptions of the surface modifications:

Sulfate (hydrophobic) Microspheres. Also called plain polystyrene microspheres, the particles are stabilized by sulfate groups. The sulfate groups are not reactive and therefore protein attachment is carried out by hydrophobic adsorption methods. The microspheres are available in a wide range of sizes. Many applications are possible, including development of rapid diagnostic tests.

Carboxyl (hydrophobic) Microspheres. These products consist of uniform surfactant-free polystyrene particles with surface carboxyl groups for charge stabilization. The microspheres are hydrophobic with carboxyl groups that are close to the surface of the particle. Although covalent coupling of substances to the particle surface is possible, these microspheres are typically used to physically adsorb proteins.

Aldehyde-modified Microspheres. The aldehyde modified microspheres are designed for coupling of molecules containing amine groups under very mild conditions (neutral pH) in a one-step process. The microspheres contain surface aldehyde groups with a density range of approximately 100 to 230 Å2 per aldehyde group. The net surface charge is negative. These microspheres can be used for many of the same applications as the carboxylate-modified products, and provide an alternate method for attaching proteins to the particle surface.

Carboxylate-modified (CML) Microspheres. These products are prepared using a copolymerization process that results in negatively charged polymer particles with a reactive carboxylated surface. Typically, the carboxyl group density ranges from about 10 to 125 Å2 per carboxyl group. The microspheres are relatively hydrophilic and the carboxyl groups can be activated using water soluble carbodimide reagents and reacted with amines to form stable covalent amide bonds. This method of conjugation is widely used to attach antibodies to the microspheres for use in diagnostic tests.

These products can be dyed with your choice of high intensity colored or fluorescent dyes. Please contact us for more information.
 Undyed Reagent Microspheres
 Product# Price  Particle Diameter  Parking Area A²/group  Surface Modification  
           
 W010PA      -    0.10 μm N/A Sulfate   SELECT  
 W010PB      -    0.10 μm N/A Sulfate   SELECT  
 W020PA      -    0.18 μm N/A Sulfate   SELECT  
 W020PB      -    0.18 μm N/A Sulfate   SELECT  
 W030PA      -    0.32 μm N/A Sulfate   SELECT  
 W030PB      -    0.32 μm N/A Sulfate   SELECT  
 W040PA      -    0.38 μm N/A Sulfate   SELECT  
 W040PB      -    0.38 μm N/A Sulfate   SELECT  
 W050PA      -    0.51 μm N/A Sulfate   SELECT  
 W050PB      -    0.51 μm N/A Sulfate   SELECT  
 W060PA      -    0.61 μm N/A Sulfate   SELECT  
 W060PB      -    0.61 μm N/A Sulfate   SELECT  
 W080PA      -    0.78 μm N/A Sulfate   SELECT  
 W080PB      -    0.78 μm N/A Sulfate   SELECT  
 W120PA      -    1.2 μm N/A Sulfate   SELECT  
 W120PB      -    1.2 μm N/A Sulfate   SELECT  
 W010HA      -    0.10 μm 175 Carboxyl   SELECT  
 W010HB      -    0.10 μm 175 Carboxyl   SELECT  
 W020HA      -    0.19 μm 192 Carboxyl   SELECT  
 W020HB      -    0.19 μm 192 Carboxyl   SELECT  
 W030HA      -    0.29 μm 148 Carboxyl   SELECT  
 W030HB      -    0.29 μm 148 Carboxyl   SELECT  
 W040HA      -    0.39 μm 102 Carboxyl   SELECT  
 W040HB      -    0.39 μm 102 Carboxyl   SELECT  
 W010LA      -    0.10 μm 107 Aldehyde-modified   SELECT  
 W010LB      -    0.10 μm 107 Aldehyde-modified   SELECT  
 W020LA      -    0.19 μm 166 Aldehyde-modified   SELECT  
 W020LB      -    0.19 μm 166 Aldehyde-modified   SELECT  
 W030LA      -    0.32 μm 227 Aldehyde-modified   SELECT  
 W030LB      -    0.32 μm 227 Aldehyde-modified   SELECT  
 W040LA      -    0.39 μm 121 Aldehyde-modified   SELECT  
 W040LB      -    0.39 μm 121 Aldehyde-modified   SELECT  
 W080LA      -    0.80 μm 80 Aldehyde-modified   SELECT  
 W080LB      -    0.80 μm 80 Aldehyde-modified   SELECT  
 W004CA      -    0.04 μm 100 Carboxylate-modified   SELECT  
 W004CB      -    0.04 μm 100 Carboxylate-modified   SELECT  
 W007CA      -    0.07 μm 85 Carboxylate-modified   SELECT  
 W007CB      -    0.07 μm 85 Carboxylate-modified   SELECT  
 W010CA      -    0.10 μm 276 Carboxylate-modified   SELECT  
 W010CB      -    0.10 μm 276 Carboxylate-modified   SELECT  
 W020CA      -    0.20 μm 45 Carboxylate-modified   SELECT  
 W020CB      -    0.20 μm 45 Carboxylate-modified   SELECT  
 W025CA      -    0.25 μm 56 Carboxylate-modified   SELECT  
 W025CB      -    0.25 μm 56 Carboxylate-modified   SELECT  
 W030CA      -    0.32 μm 64 Carboxylate-modified   SELECT  
 W030CB      -    0.32 μm 64 Carboxylate-modified   SELECT  
 W040CA      -    0.39 μm 46 Carboxylate-modified   SELECT  
 W040CB      -    0.39 μm 46 Carboxylate-modified   SELECT  
 W050CA      -    0.51 μm 39 Carboxylate-modified   SELECT  
 W050CB      -    0.51 μm 39 Carboxylate-modified   SELECT  
 W080CA      -    0.78 μm 51 Carboxylate-modified   SELECT  
 W080CB      -    0.78 μm 51 Carboxylate-modified   SELECT  
 W090CA      -    0.95 μm 8 Carboxylate-modified   SELECT  
 W090CB      -    0.95 μm 8 Carboxylate-modified   SELECT  
 W400CA      -    3.6 μm 7 Carboxylate-modified   SELECT  
 W400CB      -    3.6 μm 7 Carboxylate-modified   SELECT  
 W500CA      -    4.6 μm 5.3 Carboxylate-modified   SELECT  
 W500CB      -    4.6 μm 5.3 Carboxylate-modified   SELECT  
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Specifications:
Particle Composition :Polystyrene or Polystyrene with coploymer grafted surface
Surface Functionalities :Sulfate
Carboxyl
Aldehyde-modified
Carboxylate-modified
Particle Size :Varies
Uniformity :< 15% CV
Concentration :4% by weight
Particle Density :1.05 g/cm³
Fill Volume :15 mL or 100 mL bottles
Content :Polymer microspheres in water
Additives :Trace amount of surfactant to inhibit agglomeration and promote stability in sizes < 0.10 µm
Expiration Date :≥ 24 months
Package Includes :Certificate of Analysis,
Material Safety Data Sheet (MSDS),
Tn-013 - surface Properties & Conjugation Methods
Storage & Handling :Refrigerate when not in use, do not freeze. Store upright and keep bottle tightly sealed. Thoroughly disperse prior to use per insert .