Proteins can be covalently modified in many ways to suit the purpose of a particular assay. A wide variety of immunological and other protein methodologies involve the use of labeled antibodies or other proteins. Enzymes, biotin, fluorophores and radioactive isotopes are commonly used in biotechnology applications to provide a detection signal. They can be conjugated to antibodies, avidin, streptavidin, Fc binding proteins such as Protein A or G, or other proteins. The labeled molecule can then be used in a variety of detection systems.
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Biotin Labeling and Detection
Pierce, the leader in avidin-biotin chemistry offers a full-range of biotinylation reagents to target a variety of functional groups for specific labeling of proteins, peptides and other biomolecules.
Fluorescent Labeling of Antibodies and Proteins
Convenient kits and activated fluorescent dyes for labeling amines, sulfhydryls and other functional groups with FITC, fluorescein, rhodamine or DyLight Fluor alternatives to Cy Dyes and Alexa Fluors.