HMDS:TMCS:Pyridine (2:1:10) reagent-catalyst-solvent formulation for one-step derivatizations
Derivatizes carbohydrates, phenols, steroids, sterols, organic acids, alcohols and some amines
Applications
The Pierce Tri-Sil Reagent is composed of HMDS, TMCS and high purity pyridine. It is useful for rapid production of TMS derivatives of polar compounds for gas chromatographic determination and biochemical synthesis. Tri-Sil Reagent is ideal for GC determination of the following:
Sugars1,6
Alcohols1
Phenols7
Steroids8,9
Sterols10,11
Bile acids and other organic acids12-14
Some amines15-17
Tri-Sil Reagent is based on the procedure of Sweeley, et al.1 and is used for the optimal conversion of organic hydroxyland polyhydroxyl compounds into TMS ethers. The versatility, speed and ease of use of our Tri-Sil Reagent has made it the most widely used silylation formulation available.
Note: Not recommended for 3-ketosteroids
References
1.Sweeley, C.C., et al. (1963). JACS 85: 2497. 2.Hedgely, E.V. and Overend, W.G. (1960). Chem. and Ind. (London), 378. 3.Ferrier, R.J. and Singelton, M.F. (1962). Tetrahedron 18: 1143. 4.Ferrier, R.J. (1962). Ibid. 18: 1149. 5.Sweeley, C.C. and Walker, B. (1964). Anal. Chem. 36:1461. 6.Brower, H.E., et al. (1966). Anal. Chem. 38: 362. 7.Langer, M., et al. (1958). Chem. And Ind. (London), 1664. 8.Luukkainen, T., et al. (1960). Biochim. Biophys. Acta 52: 599. 9.Miettinen, T.A., et al. (1965). J. Lipid Res. 6: 411. 10.Nair, P.P., et al. (1965). Anal. Chem. 37:631. 11.Rozanski, A. (1966). Anal. Chem. 38:36. 12.Makita, M. and Wells, W.W. (1963). Anal. Biochem. 5:523. 13.Burkhard, C.A. (1957). J. Org. Chem. 22:592. 14.Mehrotra, R.C. and Pant, B.C. (1963). J. Ind. Chem. Soc. 40:623. 15.Birkofer, L. and Ritter, A. (1960). Chem. Ber. 90: 424. 16.Horning, E.C., et al. (1964). Anal. Chem. 36: 1546. 17.Capella P. and Horning, E.C. (1966). Anal. Chem. 38: 316. 18.Betts, T.J., et al. (1984). J. Chrom. 291: 361. 19.Ng, L., et al. (1993). J. Chrom. 637: 104.
0 Items 
