Derivatizes amino acid optical isomers in just 90 minutes.
Product Detail
Highlights:
Nanomole detection
Pre-column derivatization detection
Chiral separation of amino acids
Reacts with primary amines
λmax = 340 nm, derivatives can be detected by UV at 340 nm with picomole sensitivity
Upon derivatization, separation and quantitation by reverse-phase HPLC; derivatives have an absorption coefficient of 3 x 104
References: 1.Marfey, P., et al. (1984). Carlsberg Res. Comm. 49, 585-590. 2.Marfey, P., et al. (1984). Carlsberg Res. Comm. 49, 591-596. 3.Szókán et al. (1988). Applications of Marfey’s Reagent in racemization studies of amino acids and peptides. J. Chrom. 444, 115-122. 4.Aberhart, D.J., et al. (1985). Separation by high-performance liquid chromato-graphy of (3R)- and 3(S)-ß-Leucine as diastereomeric derivatives. 151, 88-91. 5.Martinez del Pozo, et al. (1989). Stereospecificity of reactions catalyzed by bacterial D-amino transaminase. J. Biol. Chem. 264(30), 17784-17789.