6N HCl in convenient, ready-to-use ampules for amino acid analysis.
Pierce supplies a variety of products for peptide hydrolysis, amino acid analysis and HPLC separations. Constant boiling HCl is specially purified to effect peptide hydrolysis at 150°C, to ensure the integrity of your amino acid analysis data and to maximize sensitivity by eliminating interferences in your amino acid analysis.
Product Detail
Pierce Hydrochloric Acid is purified and packaged to ensure a ninhydrin negative blank on hydrolysis. Convenient, pre-scored ampule packaging of the ready-to-use HCl maintains reagent integrity. This virtually eliminates exposure to laboratory atmospheres, fingerprints and other contaminants resulting from pipetting from bulk bottles.
Eveleigh and Winter give an excellent description of the total protein hydrolysis technique using Constant Boiling Hydrochloric Acid.1 Standard protein hydrolysis conditions are 105-110°C for 16-24 hours. At 150°C, this reagent can hydrolyze peptides in 6 hours.
Constant Boiling HCl Highlights
Hydrolyzes peptides in 6 hours at 150°C
Specially purified to give a ninhydrin-negative blank on hydrolysis
Packaged in ampules to eliminate contamination and ensure product integrity
References 1.Eveleigh, J.W. and Winter, G.D. (1970). Protein Sequence Determination, Ed Needleman, S.B., Springer-Verlag, pp 92-95. 2.Blankenship, et al. (1989). High-sensitivity amino acid analysis by derivatization with o-Phthaldialdehyde and 9-Fluorescence detection: applications in protein structure determination. Anal. Biochem. 178, 227-232. 3.Hurley, J.B., et al. (1984). Isolation and characterization of a cDNA clone for the λ subunit of bovine retinal transducin. Proc. Natl. Acad. Sci. U.S.A. 81, 6948-6952. 4.Lee, K., et al. (1979). Derivatization of cysteine and cystine for fluorescence amino acid analysis with the o-Phthaldialdehyde/ 2-mercaptoethanol reagent. J. Biol. Chem. July 25, 6248-6251.
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