Preparing to analyze peptides and proteins using LC/MS? Use 1 ml Formic Acid ampules and have confidence in the integrity of your mobile-phase
High-purity Formic Acid Ampules are a convenient, contamination-free alternative for preparing elution solvents for HPLC separations of proteins and peptides. Open and use the vial when you need it, assuring that the formic acid has not been compromised.
Product Detail
Pierce high-purity formic acid is sealed in amber glass ampules under a dry nitrogen atmosphere. A pre-measured aliquot of acid greatly simplifies preparation of liter quantities of mobile phases at the standard 0.1% formic acid concentration. The quality of this formic acid coupled with ampule packaging provides reliability and convenience that adds value to both the chromatographic and MS results.
Formic Acid, Reverse-Phase HPLC and Mass Spectrometry
Formic acid is a common component of reverse-phase mobile phases that provide protons for LC/MS analysis. The presence of a low concentration of formic acid in the mobile phase is also known to improve the peak shapes of the resulting separation. Unlike trifluoroacetic acid (TFA), formic acid is not an ion-pairing agent and it does not suppress MS ionization of polypeptides when used as a mobile-phase component.
Properties of Formic Acid
Molecular Forumula:
HCOOH (CH202)
Molecular Weight:
46.02 g/mole
Density:
1.22 g/ml
CAS Number:
64-18-6
Refractive Index:
1.3701-1.3921(20ºC)
Flash Point:
69ºC
Freezing Point:
8ºC
Formic Acid Highlights
99+% pure formic acid
Consistent LC baselines
No potential interference introduced in LC or MS applications
No signal suppression in the mass spectrometer
High-performance ampule packaging
Amber glass, pre-scored, nitrogen-flushed ampules protect formic acid from light, moisture and contamination
Convenient format
Ampule packaging simplifies the preparation of gradient and isocratic mobile phases containing 0.1% (v/v) formic acid in water or acetonitrile; the contents of a single vial in a final volume of 1 L of solvent yields a mobile phase of the most common formic acid concentration.
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