Peptide Retention Standard for Reversed Phase HPLC
Increases the efficiency of peptide elution profile predictions.
Product Detail
A simple, quantitative method for predicting peptide retention times was developed by Guo, et. al.1-3 Retention times are predicted by totaling the values that represent the contribution in minutes of each amino acid residue and the peptide terminal groups.
Retention time is dependent upon the molecular weight of the peptide. The effect on retention is relatively unimportant with a small peptide, but it increases with the size of the molecule. The accuracy of predicting peptide retention time significantly decreases beyond 20 residues. To ensure accuracy, a peptide standard is used to correct for instrument variation, column aging, n-alkyl chain length variation and ligand density.
By using Pierce Peptide Retention Standard, you can:
Determine the relative order of peptide elution of a complex mixture
Increase the efficiency of predicting peptide elution profiles
Save time in peptide purification
Simplify identification of specific peptides in a complex mixture
Predict the HPLC retention time for peptides of known amino acid composition on reverse phase HPLC columns
Monitor column performance – efficiency, selectivity and resolution during column aging
Compare reverse-phase columns from different manufacturers
Evaluate reverse-phase supports of varying n-alkyl chain lengths and ligand densities
References 1.Guo, D., et al. (1985). Proceedings of the Ninth American Peptide Symposium, Published by Pierce, Rockford, Illinois, page 23. 2.Guo, D., et al. (1986). J. Chromatogr. 359, 499-517. 3.Guo, D., et al. (1986). J. Chromatogr. 359, 519-532. 4.Mant, C.T. and Hodges, R.S. (1986). L.C. Magazine Liq. Chrom. and HPLC 4(3), 250. 5.Guo, D., et al. (1987). J. Chromatogr. 386, 205-222.
Peptide Retention Standard for Reversed Phase HPLC
1 Artículos Total 7,000.00 USD 
