Solaris qPCR Gene Expression Assays

  Solaris qPCR Gene Expression Assays



Solaris qPCR Gene Expression Assays are pre-designed, gene specific probe and primer pairs that utilize minor groove binder (MGB™) and SuperBase technologies to deliver repeatable, sensitive and gene specific quantification.

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Through the use of advanced software design and probe technologies, we have adopted a novel approach to designing qPCR probe detection assays. With this novel approach, we are able to design each assay to a consensus sequence that covers all known splice variants of the target gene. This strategy allows us to recommend one optimal assay for each gene target, making it easier than ever to incorporate the benefits of qPCR probe detection assays into your workflow.

In addition to assay design advancements, Solaris qPCR Assays also offer the following benefits:

  • The sequence information is provided.  Probe and primer sequence information if provided with every assay so you can publish with confidence.
  • The master mix is blue.  Solaris assays have been developed with our optimized, blue qPCR master mix for ultimate assay performance and ease-of-use.  See your reagents as you pipette , track your progress across multiple wells, and visually assess your set-up. 
  • The protocol is familiar.  If you currently use qPCR probes assays, Solaris assays will not require major changes to your protocol and routine.
  • The result is a high performance assay. You can expect excellent results in terms of repeatability, sensitivity, dynamic range, efficiency and specificity.


FEATURES

  • MGB and Superbase probe chemistries:  High performance assays and whole genome coverage
  • One assay per gene:  Ensures the most optimal assay is selected
  • All known splice variants detected: More biologically relevant results and more confidence in your data
  • Universal thermal cycling conditions:  All assays use the same thermal cycling protocol for ease of use and time savings
  • Disclosure of assay sequence:  Improves publication quality and follows MIQE recommended guidelines
  • Complete genome coverage:  Enables the use of a single detection strategy, saving time and effort
  • Blue Solaris Master Mix:  Visual confirmation of set-up helps eliminate pipetting errors and further enhances the repeatability of your data.


KIT COMPONENTS

Each pre-designed Solaris qPCR Assay consists of a single probe and primer pair specific to your target gene.  When you order your gene specific assay, you will receive: 

  • All assay components at a 20X concentration including
     - Two primers provided at 800 nM each
     - One gene specific probe provided at 200 nM
  • The sequence information for your gene specific probe and primers
  • A high performance Solaris qPCR Gene Expression Master Mix compatible with your specific qPCR instrument model (ordered separately)

For a gene specific product search, please visit www.thermo.com/solarissearch

To learn more or purchase, please visit www.thermo.com/solaris

 

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