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  BioBasic SEC (Size Exclusion) Columns


o   Improved reproducibility, efficiency and column lifetimes
o   Range of stationary phases to optimize selectivity
o   Powerful resolution, even on short column lengths

You can now also order additional proteomics resources, or learn about other mass spectrometry or proteomics  solutions.
   Product Detail


BioBasic SEC columns are available in four pore sizes (60, 120, 300 and 1000Å) to cater for separations of samples from 100 to 10,000,000 molecular weight.  The columns are based on porous silica particles coated with with a hydrophillic polymer for superior column lifetimes. They give superior resolution with 100% aqueous or organic eluents, and do not swell or shrink with changes in solvent, showing higher peak capacityies than polymer-based SEC columns.  The polymer coating eliminates secondary interactions so that samples are separated based stricly on their size in solution. 
BioBasic SEC columns are ideal for high efficiency gel filtration separation of proteins and other biological molecules where the absence of secondary interactions such as adsorption, is essential for accurate analysis.

The columns are offered in a standard 300 x 7.8mm size, and also 150 x 7.8mm for faster separations, as well as a 30mm length guard column to maximize column lifetimes.

  

Column

Pore Size (Å) 

Molecular Weight (kDaltons)

Proteins Pullalans

Polyethylene Oxides/Glycols

 BioBasic SEC 60  60  0.1 - 6 0.3 - 6  0.1 - 4
 BioBasic SEC120  120  0.1 - 50 0.3 - 12  0.4 - 10
 BioBasic SEC 300  300  1 - 500 1 - 100  2 - 100
 BioBasic SEC 1000  1000  20 - 4000 20 - >1000 not recommended

 
 BioBasic SEC (Size Exclusion) Columns
 Product# Price  Description  
       
 73305-157846      -    150x7.8mm 5µm BioBasic SEC 60 column   SELECT  
 73405-157846      -    150x7.8mm 5µm BioBasic SEC 120 column   SELECT  
 73505-157846      -    150x7.8mm 5µm BioBasic SEC 300 column   SELECT  
 73605-157846      -    150x7.8mm 5µm BioBasic SEC 1000 column   SELECT  
 73305-307846      -    300x7.8mm 5µm BioBasic SEC 60 column   SELECT  
 73405-307846      -    300x7.8mm 5µm BioBasic SEC 120 column   SELECT  
 73505-307846      -    300x7.8mm 5µm BioBasic SEC 300 column   SELECT  
 73605-307846      -    300x7.8mm 5µm BioBasic SEC 1000 column   SELECT  
 73305-037821      -    30x7.8mm 5µm BioBasic SEC 60 guard column   SELECT  
 73405-037821      -    30x7.8mm 5µm BioBasic SEC 120 guard column   SELECT  
 73505-037821      -    30x7.8mm 5µm BioBasic SEC 300 guard column   SELECT  
 73605-037821      -    30x7.8mm 5µm BioBasic SEC 1000 guard column   SELECT  
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Postal Mail:
  • BioBasic HPLC Columns Technical Guide
  • BioBasic SEC Columns Technical Guide
  • HPLC Analysis of Biomolecules Technical Guide
  • Successful HPLC Operation: A Troubleshooting Guide (CD)
  • FREE 2008/2009 Chromatography Columns & Consumables Catalog
    • Electronic Mail:
  • Sample clean-up strategies for the LC/MS analysis of small molecules in serum (PDF)
    • Specifications:
    Available Particle Sizes (µm) :5
    Available Pore Diameters (Å) :60, 120, 300, 1000
    USP Listing L25 :BioBasic SEC 60 and 120
    USP Listing L33 :BioBasic SEC 300 and 1000
    Pore Volume BioBasic SEC 60 :0.70 cc/gram
    Pore Volume BioBasic SEC 120 :1.0 cc/gram
    Pore Volume BioBasic SEC 300 :0.90 cc/gram
    Pore Volume BioBasic SEC 1000 :0.90 cc/gram