ProSightPC 1.0 is the first stand-alone software for analyzing top-down proteomics data. This software package uses five different search modes (including Accurate Mass, Biomarker, Sequence Tag, Single Protein and Gene Restricted search modes) to determine the exact protein sequence including modifications and alternative splicing. ProSightPC uses fragmentation from ECD, IRMPD or CID to determine the protein sequence. The proprietary ProSight Warehouse allows users to import FASTA databases and shotgun annotate these databases with all possible modifications. ProSightPC also uses the Sequence Gazer to allow users to validate their search results and convince themselves they have the correct sequence.
The first stand-alone software for top down proteomics
Top down sequencing using ProSightPC provides protein sequence information including:
–Exact sites of modifications –Truncated proteins, splice variants and biomarkers –Exact confirmation of bottom-up experiments
Uses different search modes and ECD, IRMPD or CID fragmentation to determine the protein sequence
–Absolute Mass search mode –Biomarker search mode –Sequence Tag search mode –Single Protein search mode –Gene Restricted search modes
Proprietary ProSight Warehouse allows searches for all PTMs and for splice variants
–Uses shotgun annotation to find all possible PTMs –Can import any FASTA databases
Sequence Gazer functionality is a manual validation tool
–Users can probe their data and add, remove or change modifications to see if the data fits better
Designed for use with the Thermo Scientific LTQ FT and LTQ FT Ultra hybrid mass spectrometers
Helps complement bottom-up data for more complete protein ID and characterization
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