TheraPure* DNA Phosphoramidites

  TheraPure* DNA Phosphoramidites



Thermo Scientific TheraPure DNA Phosphoramidites are ideal for the most demanding applications of synthetic DNA oligonucleotides due to controlled levels of critical reactive impurities, controlled water content, and ultra high purity.

   Product Detail



  • TheraPure phosphoramidites are designed for customers that require highly defined impurity profiles and strictly controlled manufacturing processes
  • Rigorously controlled manufacturing processes minimize levels of reactive P(III) impurities which could be incorporated into the oligonucleotide product and/or result in the accumulation of by-products which can be difficult to remove during purification
  • Limited residual water improves amidite stability, eliminating the interference with oligonucleotides synthesis that can be caused by excessive water

Process and Supply Chain Control:
  • A controlled raw material supply chain and established vendor partnerships ensure consistency and security of supply
  • All TheraPure products use synthetic nucleoside sources from materials of no known animal origin
  • Processes are strictly controlled and monitored from material procurement through final QC and product release under ISO9001 standards, providing batch-to-batch reproducibility
  • All TheraPure RNA phosphoramidites are supplied with lot-specific quality control data including HPLC and phosphorous NMR analyses

Flexible Packaging:
  • Common pack sizes fit most commercially available synthesizers and are in stock for immediate delivery
  • Custom and bulk packaging, including??multikilogram lots, are available
 TheraPure* DNA Phosphoramidites
 PRODUCT# Price  Image  Description  
         
 27-2030-00      -  
 
 
 TheraPure dA Phosphoramidite; Custom pack size   SELECT  
 27-2030-06      -  
 
 
 TheraPure dA Phosphoramidite; 10g   SELECT  
 27-2032-00      -  
 
 
 TheraPure dC Phosphoramidite; Custom pack size   SELECT  
 27-2032-06      -  
 
 
 TheraPure dC Phosphoramidite; 10g   SELECT  
 27-2034-00      -  
 
 
 TheraPure dG Phosphoramidite; Custom pack size   SELECT  
 27-2034-06      -  
 
 
 TheraPure dG Phosphoramidite; 10g   SELECT  
 27-2036-00      -  
 
 
 TheraPure T Phosphoramidite; Custom pack size   SELECT  
 27-2036-06      -  
 
 
 TheraPure T Phosphoramidite; 10g   SELECT  
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Specifications:
Standard Base Protection :Benzoyl for dA and dC; Isobutyryl for dG
HPLC Single Critical Impurity :≤0.10%
Identification by UV Spectrum :From 200-350nm, the maxima and minima of the sample and standard spectra are within ±2nm
Color Intensity of Powder :Must not exceed Pantone #S1-8 or #S5-8
Purity by HPLC and 31P NMR :≥99%
Recommended Deprotection Conditions :Incubate the oligo in concentrated ammonia at 55°C for 16 hours
HPLC Sum of Critical Impurities :≤0.3%
Solution Clarity :0.2M solution in acetonitrile is free from undissolved particulate
31P NMR Purity :Sum of nonprimary peaks at 140-152ppm is ≤0.3 mol%
Sum of nonprimary peaks at 5-35ppm is ≤1.0 mol %
No peak(s) above 170ppm
Water Content :≤0.3%