TheraPure DNA Phosphoramidites

  TheraPure DNA Phosphoramidites



Controlled levels of critical reactive impurities, controlled water content and ultra high purity and make Thermo Scientific TheraPure® phosphoramidites ideal for the most demanding applications of synthetic DNA oligonucleotides.

TheraPure phosphoramidites are designed for customers that require highly defined impurity profiles and strictly controlled manufacturing processes.

   Product Detail


Low Critical Impurities
Rigorously controlled manufacturing processes minimize levels of reactive P(III) impurities which could be incorporated into the oligonucleotide product and/or result in the accumulation of by-products which can be difficult to remove during purification.

Documented Process & Supply Chain Control
Our controlled raw material supply chain and established vendor partnerships ensure consistency and security of supply.  All TheraPure products use synthetic nucleoside sources from materials of no known animal origin. Processes are strictly controlled and monitored from material procurement through final QC and product release under ISO9001 standards.  These validated processes provide batch to batch reproducibility. All TheraPure DNA phosphoramidites are supplied with lot-specific quality control data including HPLC and phosphorous NMR analyses.

Control of Residual Water
TheraPure DNA phosphoramidites feature limited residual water for improved amidite stability.  Excessive water can interfere with oligonucleotides synthesis.

Kilogram to Ton Quantities
TheraPure DNA products are available in gram to metric ton quantities. Highly scaleable amidite manufacturing facilities enable us to meet requirements for very large scale oligonucleotide synthesis.

Flexible Packaging
Common pack sizes to fit most commercially available synthesizers are in stock and ready for immediate delivery.  Custom and bulk packaging is also available for specific requirements.

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 TheraPure DNA Phosphoramidites
 Product# Price  Image  Product Name  
         
 27-2030-00      -  
 
 
 TheraPure dA Phosphoramidite,
custom pack size
  SELECT  
 27-2030-06      -  
 
 
 TheraPure dA Phosphoramidite,
10 g
  SELECT  
 27-2032-00      -  
 
 
 TheraPure dC Phosphoramidite,
custom pack size
  SELECT  
 27-2032-06      -  
 
 
 TheraPure dC Phosphoramidite,
10 g
  SELECT  
 27-2034-00      -  
 
 
 TheraPure dG Phosphoramidite,
custom pack size
  SELECT  
 27-2034-06      -  
 
 
 TheraPure dG Phosphoramidite,
10 g
  SELECT  
 27-2036-00      -  
 
 
 TheraPure T Phosphoramidite,
custom pack size
  SELECT  
 27-2036-06      -  
 
 
 TheraPure T Phosphoramidite,
10 g
  SELECT  
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Specifications:
31P NMR Purity :Sum of non-primary peaks at 140-152 ppm is ≤0.3 mol %

Sum of non-primary peaks at 5-35 ppm is ≤1.0 mol %

No peak(s) above 170 ppm

Purity by HPLC and 31P NMR :≥99%
HPLC Sum of Critical Impurities :≤0.3%
HPLC Single Critical Impurity :≤0.10%
Water content :≤0.3%
Identification by UV Spectrum :From 200-350 nm, the maxima and minima of the sample and standard spectra are within ±2nm
Solution Clarity :0.2 M solution in acetonitrile is free from undissolved particulate
Color intensity of powder :Must not exceed Pantone #S1-8 or #S5-8
Recommended Deprotection Conditions :Incubate the oligo in concentrated ammonia at 55° for 16 hours
Standard Base protection :Benozyl for dA & dC;
Isobutyryl for dG.