Thermo Scientific CytosALL Reagent is used for the rapid preparation of cytoplasmic RNA from mammalian cells grown in adherent or suspension culture.
Product Detail
Lysates prepared with CytosALL Reagent are free of significant genomic DNA contamination. Subsequent DNA removal or further RNA purification is not required prior to using the lysates in a wide range of applications including RT-PCR, QRT-PCR, complimentary DNA (cDNA) synthesis and assessment of gene knock-down efficacy by RNA interference (RNAi). The reagent is ideal for use in applications where further processing of the lysate could lead to sample loss, introduce sequence bias, or require the introduction of undesired additional steps or reagents.
Lysate is free from significant genomic DNA contamination, reducing the potential for false positive results in RT-PCR and QRT-PCR
No need for DNase inactivation that could compromise RNA quality
Standard primers can be used—cDNA-specific primers that differentiate between PCR signal derived from genomic DNA versus cDNA transcripts are unnecessary
Time, handling and cost associated with Poly (A) or silica-based separation are eliminated
Sample loss and the introduction of size or sequence bias in the RNA population due to purification are avoided
Simple Lysis Procedure:
Procedure is completed in less than 10 minutes
Lysis volume can be scaled up or down with cell number
Recommended lysis volume: 600µL reagent/106 cells
Lysates can be used immediately or stored at -20°C for later use
Procedure minimizes handling that could lead to sample contamination or loss
Simple procedure allows multiple samples to be processed simultaneously
Organic extractions are unnecessary, avoiding residual alcohol, phenol or salts that can inhibit enzymatic reactions
No hazardous waste disposal
Quality Assurance:
The quality of the Thermo Scientific CytosALL Reagent is supported by established technical expertise and documented manufacturing systems which ensure lot-to-lot quality and consistency
Processes compliant with ISO9001-2000 standards are strictly controlled and monitored from material procurement through final QC and product release
Required Accessories: Protocol requires RNase inhibitorStorage Conditions: Store at 4°C.
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