Cyto-Cal Multifluor Fluorescence Intensity Calibrator

The Cyto-Cal Multifluor Fluorescence Intensity Calibrator is designed to simultaneously monitor flow cytometer stability as well as provide a check of instrument sensitivity and performance.  Although the product can provide relative fluorescence estimations of labeled cells (MEFL), it is not designed for quantitation of fluorochromes on cells.

The calibrator is a uniform mixture of 3 µm microspheres with three dyes in seven different fluorescent intensities.  Due to the high uniformity of the microspheres, singlet gating is not required.  The calibrator consists of a single vial of fluorescent beads precisely stained with fluorescent dyes that have optimized intensity and broad emission in multiple channels (FITC, PE, PE-Cy5, PE-Texas Red, APC, APC-Cy7).  Each intensity level has an MEFL (mean equivalent fluorochrome)* value for estimation of relative fluorescence intensities of labeled cells.

*MEFL is also referred to as Molecules of Equivalent Soluble Fluorochrome (MESF).  The MEFL values provided are not traceable to a standard reference material.