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  Extensor™ Long PCR Enzyme & Master Mixes


The Thermo Scientific Extensor Long Enzyme Mix provides superior yield and sensitivity for long fragment PCR applications. Standard Taq DNA polymerase incorporates 1 error in every 9kb. When amplifying fragments that are close to 9kb or larger, it is important to ensure that you are amplifying with no errors. The Extensor Long PCR Enzyme Mix is able to amplify fragments up to and greater than 20kb with 6 times the fidelity and double the yield of Taq.
   Product Detail


Applications 

  • Long range PCR amplification of fragments >9kb
  • High fidelity PCR 
  • Amplification of difficult and GC rich fragments
  • Cloning
  • Mutagenesis 

Description

Extensor Long PCR Enzyme Mix is a blend of ThermoPrime Taq DNA Polymerase with a proprietary thermostable proofreading enzyme. The two enzymes act synergistically to generate larger products than either enzyme individually (Up to 20kb). This makes the enzyme blend ideal for the full-length amplification of long sequences for cloning. The majority of the activity in the Extensor Long PCR Enzyme Mix comes from ThermoPrime Taq DNA Polymerase. Despite the 3’ to 5’ exonuclease (proofreading) activity present in the mix, sufficient PCR products will be generated with an A-overhang for T/A cloning (Mung bean nuclease can be used on products to remove the A-overhang for blunt end cloning).

Two buffer systems are available for application specific optimization:

  • Extensor Buffer 1 is used for amplifying fragments up to 12kb.
  • Extensor Buffer 2 is used for amplicons longer than 12kb or problematic PCR.

The enzyme is available in an easy to use master mix format; the Extensor PCR Enzyme Mix, reaction buffer, dNTPs and MgCl2 are all present in the mix. Master mixes are also available in a ReddyMix format, which includes an inert red dye and precipitant for direct gel loading of the PCR product. These convenient master mix formats reduce the risk of contamination and reagent handling errors. 

Features

  • Synergistic enzyme mix to amplify DNA templates up to 20kb
  • 6 times higher fidelity than standard Taq DNA polymerase
  • Double the yields of standard Taq DNA polymerase
  • Two buffer systems for application specific optimization
  • Proofreading enzyme has 5' to 3' exonuclease activity
  • Storage at +4°C eliminates freeze/thawing of individual components
  • Available in master mix format and ReddyMix format for significant time saving during PCR setup

Kit Contents for Core Kits

  • Vial of Extensor Enzyme Blend (5U/μl)
  • Vial of Extensor 10X Buffer 1*
  • Vial of Extensor 10X Buffer 2*
  • Vial of 25mM MgCl2

* The 10X buffers both contain 22.5mM MgCl2 and dNTPs.  An additional vial of 25mM MgCl2 is provided for any optimization that may be necessary.

Also available with Lambda DNA and primers to be used as a control.

Kit Contents for master mixes

  • Vial of Extensor Long PCR Master Mix (4 versions available - see table below).

Master Mix Options Table

Cat.
No.		 Extensor Buffer ReddyMix Version? dNTPs (µM) MgCl2 (mM) Total Taq DNA Pol.
AB-0792  1   No  350 each  2.25  1.25U 
AB-0793  2  No  500 each  2.25  1.25U
AB-0794  1  Yes  350 each  2.25  1.25U
AB-0795  2  Yes  500 each  2.25  1.25U
 Extensor™ Long PCR Enzyme & Master Mixes
 Product# Price  Description  
       
 AB-0720/A      -    Enzyme w/ Buffer 1 & 2
(80 x 25μl reactions)		   SELECT  
 AB-0720/B      -    Enzyme w/ Buffer 1 & 2
(400 x 25μl reactions)		   SELECT  
 AB-0721/A      -    Enzyme w/ Buffer 1 & 2
+ Control Template & Primers
(80 x 25μl reactions)		   SELECT  
 AB-0721/B      -    Enzyme w/ Buffer 1 & 2
+ Control Template & Primers
(400 x 25μl reactions)		   SELECT  
 AB-0792/A      -    2X Master Mix containing Buffer 1
(80 x 25μl reactions)		   SELECT  
 AB-0792/B      -    2X Master Mix containing Buffer 1
(400 x 25μl reactions)		   SELECT  
 AB-0793/A      -    2X Master Mix containing Buffer 2
(80 x 25μl reactions)		   SELECT  
 AB-0793/B      -    2X Master Mix containing Buffer 2
(400 x 25μl reactions)		   SELECT  
 AB-0794/A      -    2X ReddyMix Master Mix containing Buffer 1
(80 x 25μl reactions)		   SELECT  
 AB-0794/B      -    2X ReddyMix Master Mix containing Buffer 1
(400 x 25μl reactions)		   SELECT  
 AB-0795/A      -    2X ReddyMix Master Mix containing Buffer 2
(80 x 25μl reactions)		   SELECT  
 AB-0795/B      -    2X ReddyMix Master Mix containing Buffer 2
(400 x 25μl reactions)		   SELECT  
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Specifications:
Storage :Can be stored at +4°C for up to 1 month. Keep at -20°C for long-term storage.
Handling :Avoid repeated freeze-thaw cycles.