ABsolute™ Blue QPCR Master Mixes
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  ABsolute™ Blue QPCR Master Mixes


Thermo Scientific ABsolute Blue QPCR Master Mixes are a range of high performance mixes specially formulated to achieve the most reproducible, sensitive and specific QPCR results.
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ABsolute Blue QPCR Master Mixes are designed to achieve the most consistent and reproducible data across all QPCR platforms. Proprietary additives give enhanced and consistent end point readings as well as low Ct values. All mixes contain Thermo-Start Taq DNA Polymerase, for increased specificity, and contain dTTP for increased reaction efficiency and sensitivity (low Ct). ABsolute Blue Master Mixes are ideal for QPCR analysis in molecular biology applications, including gene expression and SNP typing.

ABsolute Blue QPCR Master Mixes contain an inert blue dye to significantly enhance the contrast between reagent and plastic. The blue color does not effect the QPCR reaction and offers an immediate check that a well has not been skipped or miss-aliquoted when dispensing manually. Also, a visual scan across the strip or plate is especially useful for a rapid validity check when using an automated dispensing system, where variation in color may indicate a blockage in one or more of the dispensing channels.

ABsolute Blue QPCR mixes are ideal for use with white reaction plates and tubes. An increase in detection efficiency is seen when using ABgene white PCR plates for QPCR. ABgene opaque white plastics reflect the fluorescence signal back to the detector more efficiently than clear plastics or white plastics from other manufacturers. While white plastics give superior QPCR results, they can make traditional colorless reagents difficult to see. With ABsolute Blue, a simple glance ensures that the correct amount of master mix has been added.

Features
  • Easy-to-see mix.  Master mix contains an inert blue dye for easy sample visualization during reaction set-up.
  • Maximum specificity.  Chemically-modified Thermo-Start Taq remains completely inactive until heated to 95ºC for 15 minutes, thereby preventing non-specific amplification.
  • Improved efficiency. The use of dTTP in place of dUTP increases reaction efficiency by approximately 5% and therefore reduces reaction time.
  • Increased sensitivity. The combination of ThermoStart Taq and dTTP increases sensitivity and allows for detection of low copy number templates.
  • Instrument specific. Individually optimized QPCR mixes designed for all major QPCR instruments. Mixes are also available for use with probes or SYBR Green chemistry.
  • Reproducible results.  All mixes undergo extensive quality control testing to ensure batch-to-batch consistency.
  • Simple protocol.  Master mix format reduces protocol steps and minimizes risk of pipetting errors and sample contamination.
Kit Components:
  • ABsolute Blue QPCR Buffer, including:
    • Thermo-Start DNA Polymerase
    • High performance dNTPs
    • MgCl2
    • SYBR Green I (only in SYBR Green mixes)
    • ROX Passive Reference Dye (in select mixes)
  • Separate MgCl2 vial for further MgCl2 optimizaton (only in SYBR Green kits)
  • Separate ROX vial (in select kits)

QPCR reagent selection guide
 ABsolute™ Blue QPCR Master Mixes
 Product# Price  Quick Description  25μl Reactions  
         
 AB-4136/A      -    + ROX Vial 200   SELECT  
 AB-4136/B      -    + ROX Vial 1600   SELECT  
 AB-4137/A      -    + ROX Vial 400   SELECT  
 AB-4137/B      -    + ROX Vial 4000   SELECT  
 AB-4138/A      -    ROX Mix 200   SELECT  
 AB-4138/B      -    ROX Mix 1600   SELECT  
 AB-4139/A      -    ROX Mix 400   SELECT  
 AB-4139/B      -    ROX Mix 4000   SELECT  
 AB-4318/A      -    Low ROX Mix 200   SELECT  
 AB-4318/B      -    Low ROX Mix 1600   SELECT  
 AB-4319/A      -    Low ROX Mix 400   SELECT  
 AB-4319/B      -    Low ROX Mix 4000   SELECT  
 AB-4166/A      -    SYBR Green + ROX Vial 200   SELECT  
 AB-4166/B      -    SYBR Green + ROX Vial 1600   SELECT  
 AB-4167/A      -    SYBR Green + ROX Vial  400   SELECT  
 AB-4167/B      -    SYBR Green + ROX Vial  4000   SELECT  
 AB-4162/A      -    SYBR Green ROX Mix  200   SELECT  
 AB-4162/B      -    SYBR Green ROX Mix 1600   SELECT  
 AB-4163/A      -    SYBR Green ROX Mix 400   SELECT  
 AB-4163/B      -    SYBR Green ROX Mix 4000   SELECT  
 AB-4322/A      -    SYBR Green Low ROX Mix 200   SELECT  
 AB-4322/B      -    SYBR Green Low ROX Mix 1600   SELECT  
 AB-4323/A      -    SYBR Green Low ROX Mix 400   SELECT  
 AB-4323/B      -    SYBR Green Low ROX Mix 4000   SELECT  
 AB-4219/A      -    SYBR Green Fluo Mix 200   SELECT  
 AB-4219/B      -    SYBR Green Fluo Mix 1600   SELECT  
 AB-4220/A      -    SYBR Green Fluo Mix 400   SELECT  
 AB-4220/B      -    SYBR Green Fluo Mix 4000   SELECT  
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Specifications:
Store at :-20°C
Handling :Avoid repeated freeze-thaw cycles. Protect from light.