In many flow cytometry applications, it is critical to be able to separate dimly labeled cells from unlabeled (autofluorescent) cells. Cyto-Cal™ Low Intensity Calibrator enables simultaneous monitoring of FL1 (FITC), FL2 (PE), FL3 (PE-Cy5) and FL4 (APC) channels for adequate fluorescence sensitivity and discrimination.
Product Detail
The calibrator contains an equal mixture of 6 micrometer (µm) undyed microspheres and microspheres dimly labeled with green, orange and red fluorescent dyes which are designed to imitate a mixture of unlabeled and dim labeled cells. The dyes are incorporated within the polymer matrix (hard-dyed) for long term stability using a proprietary Firefli™ process.