Fluorescent microspheres are hard-dyed (internally-dyed) polymer beads which utilize the Firefli™ process to incorporate the dye throughout the polymer matrix. This method produces bright fluorescent colors, minimizes photobleaching and prevents dye leaching into aqueous media. The microspheres are made of polystyrene (PS), which has a density of 1.05 g/cm3 and a refractive index of 1.59 @ 589nm (25°C). The aqueous suspensions are packaged as 1% solids.
These microspheres can be detected with an epifluorescence microscope, confocal microscope, fluorometer, fluorescence spectrophotometer, or fluorescence activated cell sorter. In addition, all of these fluorescent microspheres can be detected using a mineral light or black light (UV).
Spectral information is approximate and for reference only. The spectral properties of the dyes are dependent on their concentration and physical environment. The exact excitation and emission maxima may vary, depending on the size and composition of the microspheres.