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  Cytotoxicity 2 Multiparameter Kit


The Cytotoxicity 2 (multiparameter) HCS Reagent Kit contains optimized reagents for simultaneous detection and quantitation in the same cell of changes in nuclear morphology and size, the cell membrane’s permeability status, changes in cell density (number of cells per field) from compound toxicity, and changes induced to EITHER the lysosome’s mass or pH OR the mitochondria’s transmembrane potential.  For these indicators of cell health, the supplied fluorescent reagents enable a fixed end-point assay in cells grown on standard high-density microplates.

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Cytotoxicity 2 Multiparameter Kit Features
  • Available in multiplex or singleplex configurations
  • Optimized protocol requiring no cell lysis, purification or filtration steps
  • Immunofluorescence-based assay provides sensitive results without radioactivity
  • Cells prepared with the HCS Reagent Kit may be analyzed directly via standard fluorescence microscopy; or HCS imaging systems
  • Assay is versatile and widely applicable to a variety of cell types

Three kit versions are available in two sizes. One version contains fluorescent probes for nuclear morphology/size (blue fluorescence), cell membrane’s permeability status (green fluorescence), lysosome mass or pH (orange fluorescence), and mitochondrial transmembrane potential (orange fluorescence). Because the probes for lysosomal mass and mitochondrial potential have similar spectra, this kit can be used to measure either organelle’s properties, depending on which fluorescent probe is used. The other two versions have, in addition to the nuclear and cell permeability probes, either the lysosomal or the mitochondrial fluorescent probe.

These reagents have been optimized for use with the ArrayScan® HCS Reader and the Cell Health Profiling BioApplication. These kits can also be used with other Cellomics BioApplication Modules. Thus, automated plate handling, focusing, image acquisition and processing, and data analysis and management are combined in one high content screening (HCS) system to assay for compound toxicity. The kit also can be used as a cell health assay to quantify the number and percentage of cells in the population beyond the normal range. Additionally, compounds can be identified that affect cell population density. Cells labeled by reagents in this kit also can be viewed and analyzed by other fluorescence microscopy-based instruments.

Cytotoxicity 2 Multiparameter Kits Figure 1

Figure 1. Staining for nuclei, cell membrane permeability and mitochondrial transmembrane potential in HepG2 cells treated with media only or with 120 µM valinomycin for 24 hours. The cells were imaged using a Cellomics ArrayScan® HCS Reader.

Cytotoxicity 2 Multiparameter Kit Figure 2

Figure 2. Staining for nuclei, cell membrane permeability and lysosomal mass/pH in HepG2 cells treated with media only or with 100 µM tacrine for 24 hours. The cells were imaged using a Cellomics ArrayScan HCS Reader.

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 Cytotoxicity 2 Multiparameter Kit
 Product# Price  Product Name  Pack Size  
         
 8400001      -    Cytotoxicity 2 (multiparameter with Lysosomal and Mitochondrial Probes)  1 x 96   SELECT  
 8400002      -    Cytotoxicity 2 (multiparameter with Lysosomal and Mitochondrial Probes) 5 x 96   SELECT  
 8400101      -    Cytotoxicity 2 (multiparameter with Lysosomal Probe) 1 x 96   SELECT  
 8400102      -    Cytotoxicity 2 (multiparameter with Lysosomal Probe) 5 x 96   SELECT  
 8400201      -    Cytotoxicity 2 (multiparameter with Mitochondrial Probe) 1 x 96   SELECT  
 8400202      -    Cytotoxicity 2 (multiparameter with Mitochondrial Probe) 5 x 96   SELECT  
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